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Applied and Environmental Microbiology
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Microorganism-Plant Interactions

Pyrrolnitrin Production by Biological Control Agent Pseudomonas cepacia B37w in Culture and in Colonized Wounds of Potatoes

Karen D. Burkhead, David A. Schisler, Patricia J. Slininger
Karen D. Burkhead
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David A. Schisler
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Patricia J. Slininger
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ABSTRACT

Bacterial strain B37w (= NRRL B-14858), an isolate noteworthy because it inhibits the growth of the bioherbicide fungus Colletotrichum truncatum, was selected for further studies of bacterial antifungal properties. This isolate was identified as a Pseudomonas cepacia strain by performing carbohydrate utilization and fatty acid profile analyses, as well as other biochemical and physiological tests. Petri plate assays revealed that strain B37w exhibited antifungal activity against the potato dry rot fungus Fusarium sambucinum. Using bioautography, we correlated antifungal activity with production of a specific compound. Isolation from strain B37w and identification of the antifungal antibiotic pyrrolnitrin are described. A whole-potato assay revealed B37w's ability to colonize potato wounds. Wounded potatoes were inoculated with B37w, and pyrrolnitrin was detected in these potatoes by thin-layer chromatography-bioautography at a concentration on the order of nanograms per wound. We performed an assay in which we examined efficacy against F. sambucinum-incited potato dry rot and found that B37w inhibited disease development. This is the first report of P. cepacia or pyrrolnitrin activity against the economically important potato pathogen F. sambucinum.

FOOTNOTES

  • ↵* Corresponding author. Mailing address: Fermentation Biochemistry Research, USDA/ARS/NCAUR, 1815 N. University St., Peoria, IL 61604. Phone: (309) 681-6287. Fax: (309) 681-6686.

  • Copyright © 1994, American Society for Microbiology
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Pyrrolnitrin Production by Biological Control Agent Pseudomonas cepacia B37w in Culture and in Colonized Wounds of Potatoes
Karen D. Burkhead, David A. Schisler, Patricia J. Slininger
Applied and Environmental Microbiology Jun 1994, 60 (6) 2031-2039; DOI:

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Pyrrolnitrin Production by Biological Control Agent Pseudomonas cepacia B37w in Culture and in Colonized Wounds of Potatoes
Karen D. Burkhead, David A. Schisler, Patricia J. Slininger
Applied and Environmental Microbiology Jun 1994, 60 (6) 2031-2039; DOI:
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