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Applied and Environmental Microbiology
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Comparative Study | Journal Article

Comparison of PCR and cell culture for detection of enteroviruses in sludge-amended field soils and determination of their transport.

T M Straub, I L Pepper, C P Gerba
T M Straub
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I L Pepper
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C P Gerba
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ABSTRACT

PCR and cell culture assays for enteroviruses were conducted on soil samples collected from an experimental farm that had received mesophilic anaerobically digested sludge for the past 7 years. Of 24 samples assayed, 21 samples were positive by PCR, implying that at least some viral nucleic acid sequences remained intact. However, these viral particles were unable to infect the Buffalo Green Monkey cell line used in subsequent cell culture assays. It is significant that positive PCR detection of nucleic acid sequences occurred even though the most recent sludge application was 3 months prior to soil sampling. Viral nucleic acid sequences were detected by PCR at points vertically and laterally displaced from sludge injections, illustrating significant transport of viruses. Rainfall and irrigation events may have contributed to viral transport.

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Comparison of PCR and cell culture for detection of enteroviruses in sludge-amended field soils and determination of their transport.
T M Straub, I L Pepper, C P Gerba
Applied and Environmental Microbiology May 1995, 61 (5) 2066-2068; DOI:

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Comparison of PCR and cell culture for detection of enteroviruses in sludge-amended field soils and determination of their transport.
T M Straub, I L Pepper, C P Gerba
Applied and Environmental Microbiology May 1995, 61 (5) 2066-2068; DOI:
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