Article Figures & Data
Figures
- Fig. 1.
Atrazine catabolic pathway identified inPseudomonas sp. strain ADP, showing the first three enzymatic reactions encoded by atzABC (7, 17, 32).
- Fig. 2.
Resting-cell suspensions of C. michiganeseATZ1 with uniformly ring-labeled [14C]atrazine and analysis of culture filtrates by HPLC, as described in Materials and Methods. The materials analyzed were atrazine (▪), hydroxyatrazine (▴),14CO2 (•), and an unknown metabolite (▾).
- Fig. 3.
Identification of the accumulating unknown metabolite produced by C. michiganese ATZ1 asN-ethylammelide by HPLC and mass spectrometry. (A) Authentic standards N-isopropylammelide andN-ethylammelide. (B) Unknown metabolite by HPLC, with its mass spectrum shown in the inset.
- Fig. 4.
Transformation of N-ethylammelide and cyanuric acid by cell extracts from Pseudomonas sp. strain CN1, as demonstrated by HPLC.
- Fig. 5.
Comparison of C. michiganese growth (▵) and atrazine catabolism (○) with consortium growth (▴) and atrazine catabolism (•) on MS medium with glucose and atrazine.
- Fig. 6.
Atrazine catabolism by the atrazine-degrading consortium, with the individual roles of consortium members indicated.
Tables
- Table 2.
Catabolism of atrazine and its metabolites by pure cultures of strains ATZ1 and CN1 and the consortium
Strain(s) Growth ona: Atrazine Hydroxyatrazine N-Ethylammelide Cyanuric acid Ethylamine Isopropylamine C. michiganeseATZ1 +* + − − + +* Pseudomonas sp. strain CN1 − NDb + + + + C. michiganese ATZ1 + Pseudomonas sp. strain CN1 +* + + + + +* Consortium +* + + + +* +*
