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Methods

A Highly Selective PCR Protocol for Detecting 16S rRNA Genes of the Genus Pseudomonas (Sensu Stricto) in Environmental Samples

Franco Widmer, Ramon J. Seidler, Patrick M. Gillevet, Lidia S. Watrud, George D. Di Giovanni
Franco Widmer
National Research Council and
National Health and Ecological Effects Research Laboratory,U.S. Environmental Protection Agency, Western Ecology Division, Corvallis, Oregon 97333, and
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Ramon J. Seidler
National Health and Ecological Effects Research Laboratory,U.S. Environmental Protection Agency, Western Ecology Division, Corvallis, Oregon 97333, and
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Patrick M. Gillevet
Institute for Bioscience, Bioinformatics, and Biotechnology, George Mason University, Manassas, Virginia 20110
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Lidia S. Watrud
National Health and Ecological Effects Research Laboratory,U.S. Environmental Protection Agency, Western Ecology Division, Corvallis, Oregon 97333, and
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George D. Di Giovanni
National Research Council and
National Health and Ecological Effects Research Laboratory,U.S. Environmental Protection Agency, Western Ecology Division, Corvallis, Oregon 97333, and
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DOI: 10.1128/AEM.64.7.2545-2553.1998
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Article Information

DOI 
https://doi.org/10.1128/AEM.64.7.2545-2553.1998
PubMed 
9647828

Published By 
American Society for Microbiology Journals
History 
  • Received June 5, 1997
  • Accepted April 9, 1998
  • Published online July 1, 1998.

Copyright & Usage 
Copyright © 1998 American Society for Microbiology

Author Information

  1. Franco Widmer1,2†,
  2. Ramon J. Seidler2*,
  3. Patrick M. Gillevet3,
  4. Lidia S. Watrud2 and
  5. George D. Di Giovanni1,2‡
  1. National Research Council1 and
  2. National Health and Ecological Effects Research Laboratory,2U.S. Environmental Protection Agency, Western Ecology Division, Corvallis, Oregon 97333, and
  3. Institute for Bioscience, Bioinformatics, and Biotechnology, George Mason University, Manassas, Virginia 201103
  • ↵* Corresponding author. Mailing address: U.S. Environmental Protection Agency, WED, NHEERL, 200 SW 35th St., Corvallis, OR 97333-4902. Phone: (541) 754-4708. Fax: (541) 754-4799. E-mail: seidler{at}mail.cor.epa.gov.

  • ↵† Present address: Institute for Terrestrial Ecology, ETH Zürich, CH-8952 Schlieren, Switzerland.; ‡ Present address: American Waterworks Services Company, Inc., Quality Control and Research Laboratory, Belleville, IL 62220.

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A Highly Selective PCR Protocol for Detecting 16S rRNA Genes of the Genus Pseudomonas (Sensu Stricto) in Environmental Samples
Franco Widmer, Ramon J. Seidler, Patrick M. Gillevet, Lidia S. Watrud, George D. Di Giovanni
Applied and Environmental Microbiology Jul 1998, 64 (7) 2545-2553; DOI: 10.1128/AEM.64.7.2545-2553.1998

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A Highly Selective PCR Protocol for Detecting 16S rRNA Genes of the Genus Pseudomonas (Sensu Stricto) in Environmental Samples
Franco Widmer, Ramon J. Seidler, Patrick M. Gillevet, Lidia S. Watrud, George D. Di Giovanni
Applied and Environmental Microbiology Jul 1998, 64 (7) 2545-2553; DOI: 10.1128/AEM.64.7.2545-2553.1998
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KEYWORDS

DNA, Bacterial
Polymerase Chain Reaction
Pseudomonas
RNA, Ribosomal, 16S

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