A Highly Selective PCR Protocol for Detecting 16S rRNA Genes of the Genus Pseudomonas (Sensu Stricto)  in Environmental Samples

Franco Widmer; Ramon J. Seidler; Patrick M. Gillevet; Lidia S. Watrud; George D. Di Giovanni

DOI:

Article Information

PubMed

9647828

Published By

American Society for Microbiology Journals

History

Received June 5, 1997
Accepted April 9, 1998
Published online July 1, 1998.

Author Information

Franco Widmer1,2 ^†,
Ramon J. Seidler2 ^*,
Patrick M. Gillevet3,
Lidia S. Watrud2 and
George D. Di Giovanni1,2 ^‡

National Research Council¹ and
National Health and Ecological Effects Research Laboratory,²U.S. Environmental Protection Agency, Western Ecology Division, Corvallis, Oregon 97333, and
Institute for Bioscience, Bioinformatics, and Biotechnology, George Mason University, Manassas, Virginia 20110³

↵* Corresponding author. Mailing address: U.S. Environmental Protection Agency, WED, NHEERL, 200 SW 35th St., Corvallis, OR 97333-4902. Phone: (541) 754-4708. Fax: (541) 754-4799. E-mail: seidler{at}mail.cor.epa.gov.
↵† Present address: Institute for Terrestrial Ecology, ETH Zürich, CH-8952 Schlieren, Switzerland.
↵‡ Present address: American Waterworks Services Company, Inc., Quality Control and Research Laboratory, Belleville, IL 62220.

View Abstract

Article usage

Article usage: January 1999 to February 2020

Rendering graph...

	Abstract	Full	Pdf
Jan 1999	16	44	9
Feb 1999	15	12	2
Mar 1999	19	12	2
Apr 1999	11	3	0
May 1999	8	9	2
Jun 1999	9	4	5
Jul 1999	16	5	5
Aug 1999	11	16	5
Sep 1999	22	5	10
Oct 1999	16	8	20
Nov 1999	19	6	24
Dec 1999	14	4	17
Jan 2000	14	12	30
Feb 2000	6	6	31
Mar 2000	8	10	43
Apr 2000	11	15	26
May 2000	11	15	32
Jun 2000	17	33	37
Jul 2000	23	19	17
Aug 2000	10	14	28
Sep 2000	9	9	23
Oct 2000	9	24	40
Nov 2000	12	30	32
Dec 2000	10	17	23
Jan 2001	11	15	26
Feb 2001	20	19	33
Mar 2001	25	34	45
Apr 2001	11	23	19
May 2001	12	22	28
Jun 2001	12	24	35
Jul 2001	9	19	24
Aug 2001	10	12	21
Sep 2001	18	21	40
Oct 2001	14	40	55
Nov 2001	4	25	41
Dec 2001	1	27	28
Jan 2002	10	26	37
Feb 2002	8	32	14
Mar 2002	13	50	28
Apr 2002	13	38	34
May 2002	10	31	69
Jun 2002	6	17	33
Jul 2002	9	22	59
Aug 2002	9	10	38
Sep 2002	10	14	33
Oct 2002	15	17	29
Nov 2002	8	15	47
Dec 2002	9	24	36
Jan 2003	11	20	35
Feb 2003	11	18	52
Mar 2003	13	38	39
Apr 2003	19	46	65
May 2003	12	38	69
Jun 2003	7	56	62
Jul 2003	12	40	52
Aug 2003	19	53	29
Sep 2003	19	52	37
Oct 2003	25	70	39
Nov 2003	25	46	45
Dec 2003	27	47	38
Jan 2004	18	58	43
Feb 2004	18	64	55
Mar 2004	29	75	67
Apr 2004	24	82	51
May 2004	29	72	54
Jun 2004	17	86	68
Jul 2004	20	82	54
Aug 2004	32	127	65
Sep 2004	26	105	64
Oct 2004	14	131	60
Nov 2004	31	162	76
Dec 2004	25	112	52
Jan 2005	17	153	61
Feb 2005	13	117	57
Mar 2005	12	128	56
Apr 2005	16	81	43
May 2005	18	66	43
Jun 2005	7	60	39
Jul 2005	18	84	34
Aug 2005	16	66	31
Sep 2005	12	58	37
Oct 2005	12	42	34
Nov 2005	9	105	36
Dec 2005	7	62	28
Jan 2006	13	68	30
Feb 2006	12	94	52
Mar 2006	23	50	21
Apr 2006	15	45	44
May 2006	38	42	38
Jun 2006	57	40	42
Jul 2006	46	58	49
Aug 2006	33	44	39
Sep 2006	36	44	45
Oct 2006	25	45	59
Nov 2006	33	84	41
Dec 2006	27	31	44
Jan 2007	29	45	54
Feb 2007	47	36	45
Mar 2007	37	29	69
Apr 2007	39	32	52
May 2007	45	31	61
Jun 2007	31	32	63
Jul 2007	37	36	53
Aug 2007	23	47	34
Sep 2007	26	27	43
Oct 2007	30	19	50
Nov 2007	38	38	66
Dec 2007	11	47	40
Jan 2008	27	60	38
Feb 2008	30	53	49
Mar 2008	33	65	58
Apr 2008	33	59	52
May 2008	21	46	41
Jun 2008	39	63	40
Jul 2008	26	60	55
Aug 2008	41	42	40
Sep 2008	57	26	55
Oct 2008	60	52	42
Nov 2008	44	30	38
Dec 2008	60	26	73
Jan 2009	53	26	37
Feb 2009	69	33	62
Mar 2009	44	19	39
Apr 2009	44	41	59
May 2009	41	33	46
Jun 2009	43	27	54
Jul 2009	33	27	59
Aug 2009	60	21	50
Sep 2009	32	23	31
Oct 2009	48	21	45
Nov 2009	71	18	47
Dec 2009	52	35	52
Jan 2010	64	21	58
Feb 2010	55	20	47
Mar 2010	66	15	51
Apr 2010	58	14	35
May 2010	66	20	64
Jun 2010	46	17	48
Jul 2010	51	24	47
Aug 2010	50	15	55
Sep 2010	58	36	62
Oct 2010	49	36	63
Nov 2010	65	36	79
Dec 2010	33	33	48
Jan 2011	35	26	61
Feb 2011	37	20	52
Mar 2011	46	24	77
Apr 2011	51	33	98
May 2011	52	30	52
Jun 2011	43	20	54
Jul 2011	52	25	58
Aug 2011	46	20	53
Sep 2011	47	26	52
Oct 2011	48	43	54
Nov 2011	58	28	67
Dec 2011	48	29	63
Jan 2012	53	16	33
Feb 2012	59	21	68
Mar 2012	91	26	56
Apr 2012	65	24	54
May 2012	48	32	82
Jun 2012	58	35	50
Jul 2012	80	30	56
Aug 2012	67	13	36
Sep 2012	70	24	39
Oct 2012	61	12	26
Nov 2012	68	16	42
Dec 2012	49	14	36
Jan 2013	63	21	47
Feb 2013	56	22	36
Mar 2013	49	36	54
Apr 2013	88	59	71
May 2013	61	54	51
Jun 2013	38	43	42
Jul 2013	47	55	44
Aug 2013	49	46	41
Sep 2013	65	35	45
Oct 2013	65	51	38
Nov 2013	66	50	43
Dec 2013	62	38	49
Jan 2014	66	32	38
Feb 2014	60	30	45
Mar 2014	193	47	58
Apr 2014	79	56	54
May 2014	52	39	32
Jun 2014	43	48	42
Jul 2014	58	43	38
Aug 2014	41	35	29
Sep 2014	56	40	39
Oct 2014	65	37	48
Nov 2014	95	50	38
Dec 2014	51	59	31
Jan 2015	61	45	42
Feb 2015	47	26	37
Mar 2015	42	48	35
Apr 2015	48	31	29
May 2015	45	45	32
Jun 2015	42	42	120
Jul 2015	68	28	439
Aug 2015	62	30	210
Sep 2015	69	39	38
Oct 2015	120	39	37
Nov 2015	52	55	41
Dec 2015	40	38	31
Jan 2016	39	46	30
Feb 2016	46	47	30
Mar 2016	25	43	59
Apr 2016	32	44	40
May 2016	28	64	47
Jun 2016	37	60	67
Jul 2016	28	46	57
Aug 2016	31	28	26
Sep 2016	39	42	46
Oct 2016	49	44	49
Nov 2016	40	62	27
Dec 2016	37	41	31
Jan 2017	38	30	25
Feb 2017	36	36	22
Mar 2017	35	35	22
Apr 2017	50	12	29
May 2017	50	61	43
Jun 2017	41	32	24
Jul 2017	21	40	19
Aug 2017	23	44	22
Sep 2017	41	56	29
Oct 2017	35	34	16
Nov 2017	43	39	26
Dec 2017	26	24	9
Jan 2018	32	33	19
Feb 2018	32	35	25
Mar 2018	50	47	35
Apr 2018	48	62	29
May 2018	38	29	30
Jun 2018	42	28	27
Jul 2018	19	23	15
Aug 2018	29	28	34
Sep 2018	21	11	25
Oct 2018	34	45	54
Nov 2018	37	23	37
Dec 2018	39	28	27
Jan 2019	21	26	28
Feb 2019	26	20	19
Mar 2019	17	21	23
Apr 2019	41	35	38
May 2019	37	40	49
Jun 2019	19	28	43
Jul 2019	26	37	40
Aug 2019	17	27	27
Sep 2019	16	27	21
Oct 2019	17	28	21
Nov 2019	18	34	17
Dec 2019	12	28	29
Jan 2020	19	56	35
Feb 2020	16	33	34