Article Figures & Data
Figures
- Fig. 1.
Growth characteristics of Ralstonia sp. strain RK1 on 2,6-DCP as determined with batch cultures. (A) Mineralization of 60 μM 2,6-DCP by Ralstonia sp. strain RK1 in a batch culture. Symbols: □, 2,6-DCP concentration; •, chloride ion concentration; ▴, CFU per milliliter. (B) Lag phase ofRalstonia sp. strain RK1 with increasing initial concentrations of 2,6-DCP. (C) Growth rate of Ralstonia sp. strain RK1 at different initial concentrations of 2,6-DCP. Solid line, curve obtained with the Monod equation (23) with a maximal growth rate of 0.082 h−1 and a Ksof 24.1 μM obtained by Lineweaver-Burk linearization of the first five data points; dashed line, prediction from Haldane equation (Monod with inhibition; Ki = 787 μM, as determined by linear regression of the last four data points). μ, growth rate. Error bars represent standard deviations from two cultures. The samex axis applies to panels B and C.
- Fig. 2.
Mineralization of high concentrations of 2,6-DCP in fixed-bed reactor 1. Symbols: ▴, 2,6-DCP feed concentration; ▪, 2,6-DCP effluent concentration; ◊, chloride ion effluent concentration. The arrows indicate experimental changes described in the text. d, days.
- Fig. 3.
Mineralization of low concentrations of 2,4-DCP, 2,6-DCP, and 2,4,6-TCP with short HRTs in fixed-bed reactor 2. (A) Symbols: ▪, feed concentration of chlorophenols (CP); ▴, effluent concentration of chlorophenols; ⧫, HRT. (B) Effluent concentrations of the three chlorophenol congeners. Symbols: □, 2,4,6-TCP; ▵, 2,6-DCP; ⧫, 2,4-DCP. (C) Reactor performance. Symbols: □, volumetric degradation (vol. deg.) yield; ▴, conversion coefficient [(feed concentration − outflow concentration)/feed concentration]. The same x axis applies to panels A through C. d, days; lr, liter reactor volume.
Tables
- Table 1.
Taxonomic features of genera and species related toRalstonia sp. strain RK1
Taxon Gelatin lysed Nitrate reductase Acid formed from glucose Growth on d-glucose Growth on gluconate Growth on adipate Pigment excretion Flagellum(a) Ralstonia sp. strain RK1 −b − − − + + − − Ralstonia eutrophaa − + − − + + − + Alcaligenes faecalisa − − − − − − − + Burkholderiaa d + − + + + + + Pseudomonas pickettiia − + + + + + − + - Table 2.
Species related to Ralstonia sp. strain RK1
Taxon EMBL accession no. % 16S rRNA homology with Ralstonia sp. strain RK1 Ralstonia sp. strain RK1 AJ002302 100 Alcaligenes sp. L31650 97.3 Ralstonia eutropha M32021 95.7 Pseudomonas solanacearum X67035 93.3 Pseudomonas pickettii L37367 92.7 Burkholderiasp. X92188 90.7 Alcaligenes faecalis D88008 86.8
