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Food Microbiology

Comparison of C18-Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovisin Bovine Milk Samples and Analysis of Samples by PCR

Brandon J. Cornejo, Alfredo Sahagún-Ruiz, Francisco Suárez-Güemes, Charles G. Thornton, Thomas A. Ficht, L. Garry Adams
Brandon J. Cornejo
Department of Biology, Colorado State University, Fort Collins, Colorado 80523;
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Alfredo Sahagún-Ruiz
Departamento de Microbiologı́a Inmunologı́a, Facultad de Medicina Veterinaria, Universidad Nacional Autónoma de México, México, Distrito Federal 04510, México;
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Francisco Suárez-Güemes
Departamento de Microbiologı́a Inmunologı́a, Facultad de Medicina Veterinaria, Universidad Nacional Autónoma de México, México, Distrito Federal 04510, México;
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Charles G. Thornton
Department of Molecular Biology & Genetics, Quest Diagnostics Incorporated, Baltimore, Maryland 21227; and
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Thomas A. Ficht
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4467
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L. Garry Adams
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4467
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DOI: 10.1128/AEM.64.8.3099-3101.1998
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Article Information

DOI 
https://doi.org/10.1128/AEM.64.8.3099-3101.1998
PubMed 
9687483

Published By 
American Society for Microbiology Journals
History 
  • Received January 23, 1998
  • Accepted June 3, 1998
  • Published online August 1, 1998.

Copyright & Usage 
Copyright © 1998 American Society for Microbiology

Author Information

  1. Brandon J. Cornejo1,
  2. Alfredo Sahagún-Ruiz2,
  3. Francisco Suárez-Güemes2,
  4. Charles G. Thornton3,
  5. Thomas A. Ficht4 and
  6. L. Garry Adams4*
  1. Department of Biology, Colorado State University, Fort Collins, Colorado 805231;
  2. Departamento de Microbiologı́a Inmunologı́a, Facultad de Medicina Veterinaria, Universidad Nacional Autónoma de México, México, Distrito Federal 04510, México2;
  3. Department of Molecular Biology & Genetics, Quest Diagnostics Incorporated, Baltimore, Maryland 212273; and
  4. Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-44674
  • ↵* Corresponding author. Mailing address: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-4467. Phone: (409) 862-4402. Fax: (409) 862-1088. E-mail:gadams{at}cvm.tamu.edu.

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Comparison of C18-Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovisin Bovine Milk Samples and Analysis of Samples by PCR
Brandon J. Cornejo, Alfredo Sahagún-Ruiz, Francisco Suárez-Güemes, Charles G. Thornton, Thomas A. Ficht, L. Garry Adams
Applied and Environmental Microbiology Aug 1998, 64 (8) 3099-3101; DOI: 10.1128/AEM.64.8.3099-3101.1998

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Comparison of C18-Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovisin Bovine Milk Samples and Analysis of Samples by PCR
Brandon J. Cornejo, Alfredo Sahagún-Ruiz, Francisco Suárez-Güemes, Charles G. Thornton, Thomas A. Ficht, L. Garry Adams
Applied and Environmental Microbiology Aug 1998, 64 (8) 3099-3101; DOI: 10.1128/AEM.64.8.3099-3101.1998
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KEYWORDS

Bacteriological Techniques
DNA, Bacterial
milk
Mycobacterium bovis
Polymerase Chain Reaction
Tuberculosis, Bovine

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