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- Table 1.
Comparative M. bovis IS6110 PCR results from CB-18- versus glass bead-processed milk samples collected from tuberculin skin test-positive tuberculous cattlea
Identification no.b Result Skin test Bacteriologyc Pathology PCR following processing with: Milk Tissue Grossd Acid-fast histologye CB-18 Glass beads T-6 + M. bovis M. bovis ND ND + Neg T-17 + M. bovis ND ND ND + + T-19 + Neg ND ND ND + + T-20 + Neg ND ND ND + Neg T-23 + M. bovis ND ND ND + Neg T-25 + M. bovis ND ND ND + Neg S1-80 + ZN+ M. bovis Liver and LN Liver and LN + + S1-84 + Neg Neg LN LN + Neg S1-85 + Neg Neg LN LN + + S1-88 + Neg M. bovis Lung and LN Neg + + S1-89 + Neg Neg Lung Neg + Neg S2-92 + Neg M. bovis Neg Neg + + S2-95 + Neg ZN+ LN LN + + S2-96 + Neg M. bovis LN Neg + + S2-97 + Neg M. bovis Lung and LN Lung and LN + + S2-98 + Neg ZN+ Lung Lung + + S3-102 + M. terrae Neg Neg Neg Neg Neg ↵a Abbreviations: Neg, negative; ND, not done; LN, lymph node.
↵b Cattle were derived either from Tizayuca, Hidalgo, México (T prefix on identification numbers) or from three ranches in Ciudad Obregon, Sonora, México: Los Morenos (S1 prefix), Progreso (S2 prefix), and Santa Cecilia (S3 prefix).
↵c Those cultures that yielded mycobacteria are indicated by the species identified. Other specimens were either culture positive with acid-fast bacilli present that could not be recovered by subculture (ZN+) or culture negative.
↵d Only those tissues that presented with suspicious lesions on gross examination are indicated. Other tissues either were negative or did not have postmortem examination done.
↵e Only those tissues presenting with acid-fast bacilli in the lesions are indicated. Other tissues either were negative for acid-fast bacilli or did not have postmortem examination done.
