Article Figures & Data
Figures
- Fig. 1.
Southern and Northern blot analyses ofAspergillus strains with an aflR probe. (A) Southern blot analysis of EcoRI-cut genomic DNAs from eight strains of Aspergillus. Lanes: 1, A. niger ATCC 9029; 2, A. parasiticus ATCC 24690; 3, A. parasiticus ATCC 36537; 4, A. parasiticus ATCC 56774; 5, A. parasiticus ATCC 56775; 6, A. oryzae ATCC 14895; 7, A. sojae ATCC 42251; 8, A. oryzae ATCC 16507. (B) Northern blot analysis of the same strains.
- Fig. 2.
Sequence comparison of the C termini of the AFLR homologs translated from GenBank database entries for A. flavus (L32576 [16]) and A. parasiticus (L26220 [5]) with the A. oryzae ATCC 14895 (A. oryzae 2; Y16967 [this paper]) sequence. Sequence data from PCR fragments obtained from A. oryzae CBS 108.24 (A. oryzae 1) and A. sojaeATCC 42551 are also included. The A. parasiticus AFLR sequence is modified to correct a sequencing error at base 1121, codon 378; the A. flavus AFLR is corrected at two sites as outlined in the text. Translation of the A. oryzae ATCC 14895 and A. sojae ATCC 42551 aflR sequences beyond their predicted stop codons is included for comparative purposes.
Tables
- Table 1.
Presence and expression of genes for aflatoxin biosynthesis in Aspergillus spp.
Strain Presence of: Expression of: nor-1 ver-1 omtA aflR nor-1 ver-1 omtA aflR A. niger ATCC 9029 − − − − − − − − A. parasiticus ATCC 24690 + + + + + + + + A. parasiticus ATCC 36537 + + + + + + + + A. parasiticus ATCC 56774 + + + + + + + + A. parasiticus ATCC 56775 + + + + + + + + A. oryzae ATCC 14895 + + + + − − − − A. sojae ATCC 42251 + + + + − − − − A. oryzae ATCC 16507 + + + + − − − −
