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Methods

Identification of Lactobacillus Isolates from the Gastrointestinal Tract, Silage, and Yoghurt by 16S-23S rRNA Gene Intergenic Spacer Region Sequence Comparisons

G. W. Tannock, A. Tilsala-Timisjarvi, S. Rodtong, J. Ng, K. Munro, T. Alatossava
G. W. Tannock
Department of Microbiology, University of Otago, Dunedin, New Zealand;
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A. Tilsala-Timisjarvi
Department of Biology, University of Oulu, Oulu, and
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S. Rodtong
School of Microbiology, Suranaree University of Technology, Nakhon Ratchasima, Thailand
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J. Ng
Department of Microbiology, University of Otago, Dunedin, New Zealand;
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K. Munro
Department of Microbiology, University of Otago, Dunedin, New Zealand;
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T. Alatossava
Department of Biology, University of Oulu, Oulu, and
Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, Sotkamo, Finland; and
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DOI: 10.1128/AEM.65.9.4264-4267.1999
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Tables

  • Table 1.

    Lactobacillus isolates identified on the basis of percent similarity to 16S-23S small spacer region sequences in GenBank

    IsolateSourceGenBank accession no. of 16S-23S sequenceSpecies identification based on 16S-23S sequence% Similarity of 16S-23S sequence to that of reference strains in GenBankGenBank accession no. of V2-V3 sequenceSpecies identification based on sequence of 16S V2-V3 region
    GTH1HumanAF158557L. salivarius subsp.salivarius100.0NAaNDb
    GTH2HumanAF158558UnidentifiedNAAF157030W. confusa
    GTH5HumanAF158559L. gasseri98.6AF157044L. gasseri
    GTH6HumanAF158560L. gasseri98.2AF157033L. gasseri
    GTH7HumanAF158561L. ruminis99.5NAND
    GTH8HumanAF158562UnidentifiedNAAF157045W. confusa
    GTH10HumanAF158563L. fermentum99.0NAND
    GTH13HumanAF158564L. rhamnosus99.5NAND
    GTH15HumanAF158565L. casei99.5AF157043L. casei group
    GTH17HumanAF158566L. rhamnosus99.5NAND
    GTH18HumanAF158567L. brevis98.2AF157038L. brevis
    GTH20HumanAF158568L. rhamnosus99.5NAND
    GTH22HumanAF158569UnidentifiedNAAF157037W. confusa
    GTH24HumanAF158570UnidentifiedNAAF157031W. confusa
    GTH26HumanAF158571UnidentifiedNAAF157034W. confusa
    GTH28HumanAF158572L. ruminis98.6AF157032L. ruminis
    GTH29HumanAF158573L. gasseri98.6AF157039L. gasseri
    GTH30HumanAF158574L. casei99.1AF157042L. casei group
    GTH32HumanAF158575L. rhamnosus100.0NAND
    GTH33HumanAF158576L. fermentum99.5NAND
    JNIYoghurtAF158577L. delbrueckii subsp.bulgaricus98.5AF157040L. delbrueckii
    JN2YoghurtAF158578L. delbrueckii subsp.bulgaricus99.5NAND
    JN3YoghurtAF158579L. helveticus99.5NAND
    JN4YoghurtAF158580L. delbrueckii subsp.bulgaricus98.6AF157041L. delbrueckii
    JN5YoghurtAF158581L. acidophilus100.0NAND
    JN6YoghurtAF158582L. acidophilus100.0NAND
    JN7YoghurtAF158583L. acidophilus100.0NAND
    JN8YoghurtAF158584L. helveticus99.5NAND
    JN9YoghurtAF158585L. acidophilus100.0NAND
    JN10YoghurtAF158586L. rhamnosus100.0NAND
    GTP1PigAF158587L. crispatus100.0NAND
    GTP2PigAF158588L. crispatus100.0NAND
    GTP3PigAF158589L. crispatus100.0NAND
    GTP4PigAF158590L. reuteri100.0NAND
    GTP5PigAF158591L. crispatus98.5AF157035L. crispatus/L. gallinarum
    GTP6PigAF158592L. crispatus100.0NAND
    SR1SilageAF158593L. pentosus/L. plantarum99.5NAND
    SR2SilageAF158594L. pentosus/L. plantarum97.5AF157036L. pentosus/L. plantarum
    GTR1MouseAF158595L. reuteri100.0NAND
    GTR3RatAF158596L. reuteri100.0NAND
    • ↵a NA, not applicable.

    • ↵b ND, not done.

  • Table 2.

    GenBank Lactobacillus 16S-23S intergenic spacer region sequences used to identify isolates

    SpeciesStrainaGenBank accession no.
    L. acidophilusATCC 4356TU32971
    L. brevisIFO 13110X74221
    L. caseiATCC 334TAF121200
    L. crispatusATCC 33820TAF074857
    L. curvatusDSM 20019TU97129
    L. delbrueckii subsp.bulgaricusATCC 11842TAF113602
    L. delbrueckii subsp. lactisATCC 15808U32968
    L. fermentumATCC 14931TAF080099
    L. gasseriATCC 33323TAF074859
    L. graminisDSM 20719TU97130
    L. hamsteriATCC 43851TAF113601
    L. helveticusATCC 15009TU32970
    L. johnsoniiATCC 33200TAF074860
    L. pentosusATCC 8041TU97134
    L. plantarumATCC 14917TAF080101
    L. reuteriDSM 20016TAF080100
    L. rhamnosusATCC 7469TAF121201
    L. ruminisATCC 277780TAF080103
    L. sakeATCC 15521TU97131
    L. salivarius subsp.saliciniusATCC 11742TAF080102
    L. salivarius subsp. salivariusATCC 11741TAF113600
    L. sharpeaeDSM 20505TAF074861
    L. zeaeATCC 393TAF074862
    • ↵a ATCC, American Type Culture Collection, Manassas, Va.; DSM, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany; superscripted T, type culture.

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Identification of Lactobacillus Isolates from the Gastrointestinal Tract, Silage, and Yoghurt by 16S-23S rRNA Gene Intergenic Spacer Region Sequence Comparisons
G. W. Tannock, A. Tilsala-Timisjarvi, S. Rodtong, J. Ng, K. Munro, T. Alatossava
Applied and Environmental Microbiology Sep 1999, 65 (9) 4264-4267; DOI: 10.1128/AEM.65.9.4264-4267.1999

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Identification of Lactobacillus Isolates from the Gastrointestinal Tract, Silage, and Yoghurt by 16S-23S rRNA Gene Intergenic Spacer Region Sequence Comparisons
G. W. Tannock, A. Tilsala-Timisjarvi, S. Rodtong, J. Ng, K. Munro, T. Alatossava
Applied and Environmental Microbiology Sep 1999, 65 (9) 4264-4267; DOI: 10.1128/AEM.65.9.4264-4267.1999
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KEYWORDS

DNA, Ribosomal
Digestive System
lactobacillus
silage
Yogurt

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