Article Figures & Data
Figures
- Fig. 1.
Dichotomous decision tree to determine the phylogenetic group of an E. coli strain by using the results of PCR amplification of the chuA and yjaA genes and DNA fragment TSPE4.C2.
- Fig. 2.
Triplex PCR profiles specific for E. coliphylogenetic groups. Each combination of chuA andyjaA gene and DNA fragment TSPE4.C2 amplification allowed phylogenetic group determination of a strain. Lanes 1 and 2, group A; lane 3, group B1; lanes 4 and 5, group D; lanes 6 and 7, group B2. Lane M contained markers.
Tables
- Table 1.
PCR amplification of the chuA andyjaA genes and DNA fragment TSPE4.C2 in E. colistrains from various collections according to phylogenetic group
Strain collection or isolates (no. of strains) Reference Group as determined by reference methods (no. of strains)a No. (%) of strains positive for amplification of: chuA yjaA TSPE4.C2 ECOR (68) 10 A (25) 0 18 (72)b 0 B1 (16) 0 1 (6)c 15 (94)c D (12) 12 (100) 0 2 (17)d B2 (15) 15 (100) 15 (100) 12 (80)e Neonatal meningitis (86) 4 A (5) 0 5 (100) 0 B1 (3) 0 1 (33) 2 (66) D (18) 18 (100) 0 2 (11) B2 (60) 60 (100) 60 (100) 59 (98) Verotoxin-producing E. coli O157:H7 (10) This study D (10) 10 (100) 0 0 Other clinical strains (64) This study A (12) 0 9 (75) 0 B1 (4) 0 0 4 (100) D (11) 11 (100) 0 1 (9) B2 (37) 37 (100) 37 (100) 34 (92) E. coliK-12 10 A (1) 0 1 0 E. coli J96 This study B2 (1) 1 1 0 All strains (230) A (43) 0 33 (77) 0 B1 (23) 0 2 (9) 21 (91) D (51) 51 (100) 0 5 (10) B2 (113) 113 (100) 113 (100) 105 (93) ↵a Phylogenetic groups were determined by multilocus enzyme electrophoresis or ribotyping.
↵b The strains negative for amplification ofyjaA were ECOR 4, ECOR 15, ECOR 16, ECOR 17, ECOR 22, ECOR 23, and ECOR 24.
↵c The strain negative for amplification ofyjaA and positive for amplification of TSPE4.C2 was ECOR 70.
↵d The strains positive for amplification of TSPE4.C2 were ECOR 49 and ECOR 50.
↵e The strains negative for amplification of TSPE4.C2 were ECOR 53, ECOR 59, and ECOR 60.
