PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities

Sven Becker; Peter Böger; Ralfh Oehlmann; Anneliese Ernst

doi:10.1128/AEM.66.11.4945-4953.2000

DOI: 10.1128/AEM.66.11.4945-4953.2000

Article Information

DOI

https://doi.org/10.1128/AEM.66.11.4945-4953.2000

PubMed

11055948

Published By

American Society for Microbiology Journals

History

Received April 14, 2000
Accepted September 3, 2000
Published online November 1, 2000.

Author Information

Sven Becker¹ *,
Peter Böger¹,
Ralfh Oehlmann² and
Anneliese Ernst³

Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, Constance,¹ and
PE Biosystems, Weiterstadt,² and
NIOO-Centre for Estuarine and Coastal Ecology, Yerseke, The Netherlands³

↵* Corresponding author. Mailing address: Universität Konstanz, Lehrstuhl für Physiologie und Biochemie der Pflanzen, 78457 Constance, Germany. Phone: 49-7531-883669. Fax: 49-7531-883042. E-mail:Sven.Becker{at}uni-konstanz.de.
† Publication 2667 of the NIOO-Centre for Estuarine and Coastal Ecology.

Article usage

Article usage: October 2000 to March 2021

Rendering graph...

	Abstract	Full	Pdf
Oct 2000	152	13	86
Nov 2000	416	179	187
Dec 2000	45	40	48
Jan 2001	50	53	49
Feb 2001	22	38	20
Mar 2001	28	64	15
Apr 2001	26	36	27
May 2001	14	46	86
Jun 2001	24	26	43
Jul 2001	9	19	50
Aug 2001	14	27	37
Sep 2001	11	16	30
Oct 2001	11	17	38
Nov 2001	13	21	52
Dec 2001	9	8	38
Jan 2002	6	20	44
Feb 2002	8	13	34
Mar 2002	8	38	57
Apr 2002	8	14	46
May 2002	16	33	55
Jun 2002	6	19	47
Jul 2002	9	15	47
Aug 2002	4	19	38
Sep 2002	7	29	77
Oct 2002	2	14	43
Nov 2002	5	28	67
Dec 2002	8	7	35
Jan 2003	7	24	45
Feb 2003	12	16	44
Mar 2003	9	18	47
Apr 2003	15	29	49
May 2003	15	37	49
Jun 2003	2	27	67
Jul 2003	13	35	49
Aug 2003	18	24	25
Sep 2003	15	33	60
Oct 2003	25	37	44
Nov 2003	18	46	58
Dec 2003	31	34	40
Jan 2004	25	34	29
Feb 2004	20	35	35
Mar 2004	26	45	34
Apr 2004	20	43	47
May 2004	26	53	38
Jun 2004	22	48	34
Jul 2004	25	49	25
Aug 2004	30	48	35
Sep 2004	32	65	65
Oct 2004	28	66	39
Nov 2004	29	58	29
Dec 2004	28	63	35
Jan 2005	17	63	50
Feb 2005	22	80	44
Mar 2005	31	55	41
Apr 2005	23	51	34
May 2005	31	77	36
Jun 2005	22	50	35
Jul 2005	27	44	28
Aug 2005	30	34	31
Sep 2005	14	44	30
Oct 2005	25	36	29
Nov 2005	15	39	41
Dec 2005	19	37	26
Jan 2006	19	47	20
Feb 2006	18	30	26
Mar 2006	12	28	22
Apr 2006	14	31	14
May 2006	46	24	25
Jun 2006	30	19	19
Jul 2006	23	22	30
Aug 2006	26	15	13
Sep 2006	31	30	37
Oct 2006	40	31	21
Nov 2006	36	17	19
Dec 2006	23	25	11
Jan 2007	25	30	30
Feb 2007	19	11	16
Mar 2007	21	15	13
Apr 2007	36	10	21
May 2007	23	15	27
Jun 2007	19	12	18
Jul 2007	18	11	12
Aug 2007	23	14	28
Sep 2007	21	10	21
Oct 2007	31	17	32
Nov 2007	18	17	31
Dec 2007	15	16	14
Jan 2008	23	17	12
Feb 2008	28	9	20
Mar 2008	24	14	27
Apr 2008	37	15	25
May 2008	26	16	23
Jun 2008	39	16	18
Jul 2008	18	11	14
Aug 2008	26	23	8
Sep 2008	43	10	27
Oct 2008	47	16	24
Nov 2008	47	18	28
Dec 2008	45	14	28
Jan 2009	47	17	21
Feb 2009	27	4	13
Mar 2009	47	12	31
Apr 2009	45	9	16
May 2009	36	10	15
Jun 2009	42	20	21
Jul 2009	31	13	22
Aug 2009	39	10	27
Sep 2009	27	11	15
Oct 2009	33	8	22
Nov 2009	35	12	34
Dec 2009	40	7	15
Jan 2010	30	3	20
Feb 2010	34	17	18
Mar 2010	51	8	24
Apr 2010	31	7	8
May 2010	37	4	18
Jun 2010	41	12	37
Jul 2010	36	11	11
Aug 2010	40	27	12
Sep 2010	39	6	15
Oct 2010	47	19	31
Nov 2010	35	19	17
Dec 2010	22	6	14
Jan 2011	18	8	27
Feb 2011	22	13	32
Mar 2011	56	12	15
Apr 2011	37	8	20
May 2011	33	29	44
Jun 2011	36	16	19
Jul 2011	43	22	26
Aug 2011	71	13	17
Sep 2011	27	38	14
Oct 2011	41	24	15
Nov 2011	58	17	14
Dec 2011	41	14	10
Jan 2012	52	12	18
Feb 2012	56	17	16
Mar 2012	41	10	9
Apr 2012	34	6	16
May 2012	23	12	8
Jun 2012	25	10	19
Jul 2012	31	10	14
Aug 2012	45	13	18
Sep 2012	31	8	16
Oct 2012	22	11	13
Nov 2012	23	9	11
Dec 2012	18	12	5
Jan 2013	32	15	14
Feb 2013	31	9	11
Mar 2013	24	19	9
Apr 2013	41	12	5
May 2013	18	15	8
Jun 2013	15	14	10
Jul 2013	24	4	7
Aug 2013	36	10	16
Sep 2013	30	6	6
Oct 2013	30	9	23
Nov 2013	38	15	23
Dec 2013	27	9	12
Jan 2014	37	9	7
Feb 2014	29	5	13
Mar 2014	43	9	12
Apr 2014	27	6	7
May 2014	21	7	8
Jun 2014	26	12	10
Jul 2014	54	9	7
Aug 2014	23	7	7
Sep 2014	31	4	4
Oct 2014	29	3	4
Nov 2014	40	6	1234
Dec 2014	47	5	9
Jan 2015	23	8	3
Feb 2015	17	8	9
Mar 2015	32	12	7
Apr 2015	19	12	5
May 2015	21	13	10
Jun 2015	13	14	7
Jul 2015	18	8	5
Aug 2015	36	10	13
Sep 2015	26	7	10
Oct 2015	43	8	18
Nov 2015	32	12	3
Dec 2015	20	6	7
Jan 2016	42	6	10
Feb 2016	36	6	7
Mar 2016	11	7	7
Apr 2016	18	3	5
May 2016	19	17	18
Jun 2016	23	7	17
Jul 2016	18	5	5
Aug 2016	22	7	5
Sep 2016	28	1	10
Oct 2016	30	9	23
Nov 2016	32	3	32
Dec 2016	31	5	7
Jan 2017	22	10	8
Feb 2017	15	13	11
Mar 2017	24	5	7
Apr 2017	21	6	8
May 2017	9	6	7
Jun 2017	8	4	4
Jul 2017	12	4	5
Aug 2017	31	4	10
Sep 2017	21	4	6
Oct 2017	23	12	4
Nov 2017	14	6	3
Dec 2017	16	5	6
Jan 2018	18	2	17
Feb 2018	10	7	3
Mar 2018	26	11	6
Apr 2018	14	5	8
May 2018	17	11	8
Jun 2018	5	7	8
Jul 2018	15	12	6
Aug 2018	13	10	18
Sep 2018	7	20	7
Oct 2018	10	21	4
Nov 2018	5	26	9
Dec 2018	6	8	6
Jan 2019	11	10	5
Feb 2019	8	19	13
Mar 2019	4	20	7
Apr 2019	2	16	10
May 2019	6	29	16
Jun 2019	2	13	1
Jul 2019	5	17	16
Aug 2019	6	15	4
Sep 2019	3	15	4
Oct 2019	3	42	7
Nov 2019	7	24	7
Dec 2019	2	26	6
Jan 2020	5	63	5
Feb 2020	2	33	6
Mar 2020	7	41	13
Apr 2020	4	57	13
May 2020	3	42	6
Jun 2020	2	50	7
Jul 2020	7	27	5
Aug 2020	5	18	3
Sep 2020	12	72	16
Oct 2020	9	53	15
Nov 2020	13	53	16
Dec 2020	5	50	20
Jan 2021	14	33	12
Feb 2021	13	38	9
Mar 2021	0	0	1