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Microbial Ecology

PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities

Sven Becker, Peter Böger, Ralfh Oehlmann, Anneliese Ernst
Sven Becker
Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, Constance, and
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Peter Böger
Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, Constance, and
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Ralfh Oehlmann
PE Biosystems, Weiterstadt, and
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Anneliese Ernst
NIOO-Centre for Estuarine and Coastal Ecology, Yerseke, The Netherlands
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DOI: 10.1128/AEM.66.11.4945-4953.2000
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Article Information

DOI 
https://doi.org/10.1128/AEM.66.11.4945-4953.2000
PubMed 
11055948

Published By 
American Society for Microbiology Journals
History 
  • Received April 14, 2000
  • Accepted September 3, 2000
  • Published online November 1, 2000.

Copyright & Usage 
Copyright © 2000 American Society for Microbiology

Author Information

  1. Sven Becker1*,
  2. Peter Böger1,
  3. Ralfh Oehlmann2 and
  4. Anneliese Ernst3
  1. Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, Constance,1 and
  2. PE Biosystems, Weiterstadt,2 and
  3. NIOO-Centre for Estuarine and Coastal Ecology, Yerseke, The Netherlands3
  • ↵* Corresponding author. Mailing address: Universität Konstanz, Lehrstuhl für Physiologie und Biochemie der Pflanzen, 78457 Constance, Germany. Phone: 49-7531-883669. Fax: 49-7531-883042. E-mail:Sven.Becker{at}uni-konstanz.de.

  • † Publication 2667 of the NIOO-Centre for Estuarine and Coastal Ecology.

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PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities
Sven Becker, Peter Böger, Ralfh Oehlmann, Anneliese Ernst
Applied and Environmental Microbiology Nov 2000, 66 (11) 4945-4953; DOI: 10.1128/AEM.66.11.4945-4953.2000

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PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities
Sven Becker, Peter Böger, Ralfh Oehlmann, Anneliese Ernst
Applied and Environmental Microbiology Nov 2000, 66 (11) 4945-4953; DOI: 10.1128/AEM.66.11.4945-4953.2000
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KEYWORDS

bacteria
Ecosystem
Polymerase Chain Reaction
Taq Polymerase

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