Article Figures & Data
Figures
- Fig. 1.
Dose-response curves for AcNPV-C6 and AcNPV-ST3 in second-instar M. brassicae and T. ni larvae. The equations for the lines are as follows: for T. ni, for AcNPV-C6 (dashed line), logit (mortality) = −14.02 + 2.836 [log10(concentration)], and for AcNPV-ST3 (solid line), logit (mortality) = −12.94 + 2.836 [log10(concentration)]; for M. brassicae (one solid line for both viruses), logit (mortality) = −7.812 + 1.213 [log10(concentration)] (logit is ln[p/( 1 − p )] and p is proportionate mortality). ▵, T. ni AcNPV-ST3; ▴,T. ni AcNPV-C6; ○, M. brassicae AcNPV-ST3; ●,M. brassicae AcNPV-C6.
- Fig. 2.
Time to death (means ± standard errors [SEs] [error bars]) for second-instar M. brassicae and T. niinfected with wild-type AcNPV-C6 and AcNPV-ST3. Values have been calculated from approximate LC50s, that is, 3 × 106 and 5 × 104 polyhedra/ml for M. brassicae ( n = 29 and 24 for AcNPV-C6 and AcNPV-ST3, respectively) and T. ni ( n = 15 and 18 for AcNPV-C6 and AcNPV-ST3, respectively), respectively.
- Fig. 3.
Virus yield (means ± SEs [error bars]) for second-instar M. brassicae and T. ni infected with wild-type AcNPV-C6 and AcNPV-ST3. Values have been calculated from approximate LC50s, that is, 3 × 106 and 5 × 104 polyhedra/ml for M. brassicae( n = 22 and 24 for AcNPV-C6 and AcNPV-ST3, respectively) and T. ni ( n = 6 and 11 for AcNPV-C6 and AcNPV-ST3, respectively), respectively.
- Fig. 4.
Relationship between yield per larva and time to death for second-instar M. brassicae treated with AcNPV-ST3 and AcNPV-C6 (concentration of each, 106 polyhedra/ml) and second-instar T. ni treated with AcNPV-C6 (concentration, 2 × 105 polyhedra/ml). (T. ni with AcNPV-ST3 has not been included, as the range of times to death at the virus concentration used are not sufficient to demonstrate the relationship.) The relationship for M. brassicae with AcNPV-C6 and AcNPV-ST3 can be described by the following equations: yield = 7.702 + 0.004233 × TTD − 0.08152 × LC and 7.9561 + 0.004233 × TTD − 0.08152 × LC, respectively. The relationship for T. ni with AcNPV-C6 can be described by the following equation: yield = 6.0681 + 0.017083 × TTD − 0.08152 × LC. (In the equations given above, yield is stated in log10 units, TTD is time to death, and LC is the log10 virus concentration.)
- Fig. 5.
The relationship between log10 yield per unit of larval weight and time to death for M. brassicae andT. ni challenged with AcNPV-C6 and AcNPV-ST3. (a) The relationship for M. brassicae infected with both AcNPV-C6 and AcNPV-ST3 is as follows: log10 (yield per mg of body weight) = 7.625 − 0.004256 × TTD (n = 211) . (b) The relationship for T. ni infected with AcNPV-C6 is as follows: log10 (yield per mg of body weight) = 6.269 − 0.00495 × TTD (n = 54) (dashed line). For T. ni infected with AcNPV-ST3, the relationship is as follows: log10 (yield per mg of body weight) = 6.11 − 0.00495 × TTD (n = 64) (solid line). (In these three equations, TTD is the time to death.)
