Quantitative Shedding of Two Genotypes of Cryptosporidium parvum in California Ground Squirrels (Spermophilus beecheyi)

Sample collection.Under a memorandum of understanding with the Wildlife Services Agency, U.S. Department of Agriculture Maricopa, Calif., California ground squirrels from throughout Kern County were dispatched by expert marksmen. Squirrels were collected from June 1998 through October 1998, sexed, and aged, and fecal samples were collected from the colon during necropsy (6, 20).

Enumeration of C. parvum.Enumeration of oocysts was performed as previously described (12). Percent recovery of the immunofluorescent assay was determined by spiking two negative squirrel fecal samples with wild-type dairy calfC. parvum oocysts to a concentration of 10⁴, 10⁵, and 10⁶ oocysts per g, with six replicates per squirrel per concentration, as previously described (12).

DNA extraction from C. parvum oocysts. C. parvum positive fecal samples that had >30 oocysts per smear were purified as previously described (9, 21). For DNA extraction, a 2.0-ml aliquot of sample was centrifuged at 15,800 × g for 10 min. Supernatant was discarded, and oocysts were resuspended in 500 μl of 10% commercial bleach solution and incubated at 37°C for 10 min, which was followed by the addition of 1.0 ml of 10% sodium thiosulfate. The samples were centrifuged as before, and the oocysts were resuspended in a commercial sodium phosphate-buffered solution and transferred to a FastDNA Multimix tube (Bio 101, Vista, Calif.). DNA was extracted according to the manufacturer's recommendations (FastDNA; Bio 101) with minor modifications.

DNA amplification conditions.Two procedures were used to determine the species and genotype of the isolates ofCryptosporidium. The first method was a nested PCR restriction fragment length polymorphism (RFLP) technique that targets the 18S small-subunit (SSU) rRNA gene locus ofCryptosporidium (24, 25). All procedures for the outer and inner (nested) PCR amplification have been previously described (24). The second PCR-RFLP genotyping method targeted the Cryptosporidium oocyst wall protein gene (COWP) (17). All procedures for the PCR amplification have been previously described (17). Negative and positive controls consisted of sterile H₂O and a PCR-confirmed bovineC. parvum sample, respectively.

Restriction enzyme digest.Ten-microliter aliquots of the various PCR products were electrophoresed in 2% agarose gel (Life Technologies, Gaithersburg, Md.) with a 100- to 1,500-bp DNA ladder (Promega Corporation, Madison, Wis.), with the size of the amplicons determined by a Bio-Rad Fluor-S MultiImager apparatus and Multi-Analyst, version 1.1, software (Bio-Rad, Hercules, Calif.). Ten microliters of nested PCR product from the SSU rRNA gene-based primers was digested with 10 U of VspI and SspI (Life Technologies and Boehringer Mannheim Corp. [Indianapolis, Ind.]) and 1 × buffer (Boehringer Mannheim Corp. and Life Technologies) (24). Amplicons obtained with the COWP gene-based primers were digested using 10 μl of PCR product mixed with 5 U ofRsaI and 1 × buffer (Boehringer Mannheim Corp.). Digests from both PCR methods were incubated at 37°C for 1 h, and 10 μl was electrophoresed in 2% agarose gel (Life Technologies). Gels were stained and the sizes of restriction fragments were determined as before.

Estimation of daily fecal production and percent moisture of fecal pellets.Naturally voided fecal pellets from 11 captive adult California ground squirrels were collected and weighed for a 24-h period. Squirrels had an ad libitum supply of water, oats, fresh weeds, and commercial rat chow. Fecal matter was then dried for 7 days at 70°C to determine percent moisture.

Statistical analyzes.The mean number of oocysts per gram was compared across the various sex and age groups, using a square-root transformation of oocyst counts and either a Welch modified two-sample t test with unequal variances or a one-way analysis of variance (as explained in volume 1 of Guide to Statistics, p. 52–92 [Data Analysis Products Division, MathSoft, Seattle, Wash.], 1999). The prevalence of oocyst shedding was compared across these sex, age, and sex-by-age groupings using a likelihood ratio test (C. Mehta and N. Patel, StatXact 4.0: user manual [Cytel Software Corporation, Cambridge, Mass.], 1996).

Three hundred and nine California ground squirrels from 17 geographic locations were tested for C. parvum oocysts. Sixteen percent of squirrels were shedding C. parvum oocysts (Table 1). The mean concentration of C. parvum for positive squirrels was 53,875 oocysts/g of feces and for all 309 squirrels in the study was 8,543 oocysts/g of feces. Percentages of adults and juvenile squirrels shedding oocysts were not significantly different. Males were about 1.5 times more likely than females to be shedding oocysts (two-sided P = 0.10 ), with males also shedding higher concentrations of oocysts than females (two-sided P = 0.05 ) (Table 1). Male squirrels are more likely than females to disperse from natal colonies and emigrate to move to nonnatal colonies (1, 5, 6, 10). This tendency of males to disperse to other colonies can promote the dissemination of C. parvumto nonkin squirrel populations. Given the occurrence of active infection of C. parvum (12 to 22%) among the different age and sex classes of California ground squirrels during much of the season of natal dispersal (late summer through early winter), it is likely that mixed cryptosporidial infections can occur when infected males, with their 20% prevalence of active shedding of oocysts, emigrate to move into new colonies. Neither the prevalence nor oocyst concentration was significantly different between male juveniles, male adults, female juveniles, and female adults. These oocyst concentrations were adjusted for the percent recovery of the immunofluorescent assay (Table 2).

Based on these data, the daily environmental loading rate of squirrelC. parvum can be roughly calculated as follows: mean shedding intensity × mean daily fecal output × population density (or size). Mean shedding intensity was recalculated so that each sex and age class category was equally weighted, resulting in a mean intensity of 9,426 oocysts/g of feces. Fecal output by the 11 captive squirrels was 119.4 g (wet weight) and 56.7 g (dry weight). Total squirrel biomass was 7,624.8 g (range of 550 to 915 g per squirrel), therefore, daily fecal production (wet weight) was ∼2% of body weight. The average mass of California ground squirrels is 100 to 150 g for young pups, with adult females ranging from 450 to 650 g and adult males ranging from 650 to 1,000 g (6,10). Population densities range from 8.4 to 92 adults per ha (1, 10, 14), resulting in biomass densities of 4,620 to 50,600 g/ha (based on a mean squirrel mass of 550 g). Therefore, the oocyst loading rate for ground squirrel populations was approximately 8.7 × 10⁵ oocysts/ha/day for low density populations and 9.5 × 10⁶ oocysts/ha/day for highly dense populations.

California ground squirrels in our population were infected with at least two genotypes of this protozoan. Specifically, isolates from nine squirrels were genotyped by the COWP method, resulting in an ∼550-bp amplicon and two visible fragments of 410 and 106 bp when digested withRsaI (Fig. 1). ThisC. parvum genotype is similar to isolates from both bovine and murine sources (17, 26). Using the nested PCR-RFLP technique for the SSU rRNA gene, the outer primer pair generated amplicons of 1,325 bp for 13 isolates (Fig. 1). The inner primer pair generated an amplicon of 831 bp for 11 of the 13 isolates (85%), and an amplicon of approximately 838 bp for 2 of the 13 isolates (15%). For the 11 isolates having the 831-bp internal amplicon, digestion with SspI resulted in visible fragments of 449, 254, and 119 bp. This nested PCR-RFLP pattern is consistent with previous isolations of C. parvum from bovine sources (24, 25). For the two isolates having the 838-bp internal amplicon, digestion with SspI resulted in visible fragments of 453 and 365 bp, a pattern consistent with previous isolates of C. parvum from porcine sources (24, 25). In contrast to the finding of two distinct genotypes from digestion with SspI, digestion withVspI of the 13 internal amplicons resulted in visible fragments of 628 and 102 to 104 bp, a pattern consistent with previous isolations of C. parvum from bovine sources, indicative of what is referred to as bovine B genotype (24,25). Having two genotypes of C. parvumcirculating within a single host population has also been observed for humans and cattle (2, 11, 13, 17, 24, 25). Whether mixed infections of C. parvum isolates from different squirrel colonies enhance the fitness of this protozoal parasite is unclear, but the occurrence of sexual multiplication (gametogony) byC. parvum suggests it is feasible that oocysts can be constructed from gamonts originating from different colonies and of different genotypes.

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Fig. 1.

Confirmation of species and genotyping of C. parvum oocysts from California ground squirrels through one-step PCR-RFLP and nested PCR-RFLP analysis. Lane 1, 100- to 1,500-bp markers; lane 2, 550-bp amplicon from COWP gene primers; lane 3, 410 and 106-bp fragments from COWP RFLP analysis withRsaI; lane 4, 1,325-bp amplicon from SSU rRNA primary product; lane 5, 831-bp amplicon from SSU rRNA secondary product, genotype bovine B; lane 6, 838-bp amplicon from SSU rRNA secondary product, genotype porcine; lane 7, 628-bp and 102- to 104-bp fragments from porcine genotype with VspI; lane 8, 453- and 365-bp fragments from porcine genotype with SspI; lane 9, 449-, 254-, and 119-bp fragments from genotype bovine B with SspI; lane 10, 628-bp and 102- to 104-bp fragments from genotype bovine B with VspI; lane 11, 100- to 1,500-bp markers.