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Invertebrate Microbiology

Susceptibility of a Field-Derived, Bacillus thuringiensis-Resistant Strain of Diamondback Moth to In Vitro-Activated Cry1Ac Toxin

Ali H. Sayyed, Roxani Gatsi, Thaleia Kouskoura, Denis J. Wright, Neil Crickmore
Ali H. Sayyed
Department of Biological Sciences, Imperial College of Science, Technology, and Medicine, Silwood Park, Ascot, Berkshire SL5 7PY, and
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Roxani Gatsi
School of Biological Sciences, University of Sussex, Falmer, Brighton, East Sussex BN1 9QG, United Kingdom
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Thaleia Kouskoura
School of Biological Sciences, University of Sussex, Falmer, Brighton, East Sussex BN1 9QG, United Kingdom
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Denis J. Wright
Department of Biological Sciences, Imperial College of Science, Technology, and Medicine, Silwood Park, Ascot, Berkshire SL5 7PY, and
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Neil Crickmore
School of Biological Sciences, University of Sussex, Falmer, Brighton, East Sussex BN1 9QG, United Kingdom
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DOI: 10.1128/AEM.67.9.4372-4373.2001
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  • Table 1.

    Toxicity of crystalline Cry1Ac for ROTH, UNSEL, and Cry1Ac-SEL populations of P. xylostella

    StrainForm of toxinLC50 (95% FL)aAvg slope (SE)RRb
    ROTHCrystalc0.0020 (0.0001–0.0085)0.69 (0.18)
    Spore-crystald0.0013 (0.0003–0.0039)0.85 (0.19)
    NAT spore-crystald, e0.0020 (0.0008–0.004)2.12 (0.53)
    UNSELCrystal0.0880 (0.034–0.19)1.29 (0.24)44
    Spore-crystal0.0920 (0.03–0.2)1.23 (0.24)71
    NAT spore-crystal0.1110 (0.0003–0.315)1.64 (0.61)55
    Cry1Ac-SELCrystal2.33 (0.14–29.62)0.54 (0.22)1,170
    Spore-crystal3.60 (1.1–15.0)0.81 (0.24)2,770
    NAT spore-crystal2.08 (0.56–6.01)0.64 (0.16)1,040
    • ↵a Expressed in micrograms per milliliter. At least 150 larvae (F11) were used in each experiment. LC50, 50% lethal concentration FL, fiducial limits.

    • ↵b Resistance ratio of 50% lethal concentrations for the UNSEL and Cry1Ac-SEL populations to those for the ROTH population.

    • ↵c Expressed in E. coli.

    • ↵d Expressed in B. thuringiensisIPS78/11 and applied as a spore-crystal mixture.

    • ↵e NAT, N-terminally activated toxin.

  • Table 2.

    Toxicity of in vitro solubilized and activated Cry1Ac for ROTH, UNSEL, and Cry1Ac-SEL populations of P. xylostella

    StrainForm of toxincLC50 (95% FL)aAvg slope (SE)RRb
    ROTHCrystal15 (8–64)0.69 (0.18)
    Solubilizedd10 (8–22)1.11 (0.29)
    Activatedd15 (15–45)1.18 (0.39)
    UNSELCrystal660 (255–1425)1.29 (0.24)44
    Solubilized3,150 (975–6300)2.02 (0.43)315
    Activated375 (135–750)1.99 (0.44)25
    Cry1Ac-SELCrystal17,475 (1,050–222,150)0.54 (0.22)1,170
    Solubilized>150,0000.44 (0.15)>15,000
    Activated1,410 (465–3,150)0.65 (0.14)94
    • ↵a Values are expressed as picomolar concentrations due to differences in mass between the protoxin and activated forms of Cry1Ac. At least 150 larvae (F11) were used in each experiment. LC50, 50% lethal concentration. FL, fiducial limits.

    • ↵b Resistance ratio of 50% lethal concentrations for the UNSEL and Cry1Ac-SEL populations to those for the ROTH population.

    • ↵c Expressed in E. coli.

    • ↵d Solubilized in 50 mM Na2CO3 and, where indicated, activated with 1 mg of trypsin per ml.

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Susceptibility of a Field-Derived, Bacillus thuringiensis-Resistant Strain of Diamondback Moth to In Vitro-Activated Cry1Ac Toxin
Ali H. Sayyed, Roxani Gatsi, Thaleia Kouskoura, Denis J. Wright, Neil Crickmore
Applied and Environmental Microbiology Sep 2001, 67 (9) 4372-4373; DOI: 10.1128/AEM.67.9.4372-4373.2001

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Susceptibility of a Field-Derived, Bacillus thuringiensis-Resistant Strain of Diamondback Moth to In Vitro-Activated Cry1Ac Toxin
Ali H. Sayyed, Roxani Gatsi, Thaleia Kouskoura, Denis J. Wright, Neil Crickmore
Applied and Environmental Microbiology Sep 2001, 67 (9) 4372-4373; DOI: 10.1128/AEM.67.9.4372-4373.2001
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KEYWORDS

Bacillus thuringiensis
Bacterial Proteins
Bacterial Toxins
Endotoxins
Moths

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