Article Figures & Data
Tables
- Table 1.
Toxicity of crystalline Cry1Ac for ROTH, UNSEL, and Cry1Ac-SEL populations of P. xylostella
Strain Form of toxin LC50 (95% FL)a Avg slope (SE) RRb ROTH Crystalc 0.0020 (0.0001–0.0085) 0.69 (0.18) Spore-crystald 0.0013 (0.0003–0.0039) 0.85 (0.19) NAT spore-crystald, e 0.0020 (0.0008–0.004) 2.12 (0.53) UNSEL Crystal 0.0880 (0.034–0.19) 1.29 (0.24) 44 Spore-crystal 0.0920 (0.03–0.2) 1.23 (0.24) 71 NAT spore-crystal 0.1110 (0.0003–0.315) 1.64 (0.61) 55 Cry1Ac-SEL Crystal 2.33 (0.14–29.62) 0.54 (0.22) 1,170 Spore-crystal 3.60 (1.1–15.0) 0.81 (0.24) 2,770 NAT spore-crystal 2.08 (0.56–6.01) 0.64 (0.16) 1,040 ↵a Expressed in micrograms per milliliter. At least 150 larvae (F11) were used in each experiment. LC50, 50% lethal concentration FL, fiducial limits.
↵b Resistance ratio of 50% lethal concentrations for the UNSEL and Cry1Ac-SEL populations to those for the ROTH population.
↵c Expressed in E. coli.
↵d Expressed in B. thuringiensisIPS78/11 and applied as a spore-crystal mixture.
↵e NAT, N-terminally activated toxin.
- Table 2.
Toxicity of in vitro solubilized and activated Cry1Ac for ROTH, UNSEL, and Cry1Ac-SEL populations of P. xylostella
Strain Form of toxinc LC50 (95% FL)a Avg slope (SE) RRb ROTH Crystal 15 (8–64) 0.69 (0.18) Solubilizedd 10 (8–22) 1.11 (0.29) Activatedd 15 (15–45) 1.18 (0.39) UNSEL Crystal 660 (255–1425) 1.29 (0.24) 44 Solubilized 3,150 (975–6300) 2.02 (0.43) 315 Activated 375 (135–750) 1.99 (0.44) 25 Cry1Ac-SEL Crystal 17,475 (1,050–222,150) 0.54 (0.22) 1,170 Solubilized >150,000 0.44 (0.15) >15,000 Activated 1,410 (465–3,150) 0.65 (0.14) 94 ↵a Values are expressed as picomolar concentrations due to differences in mass between the protoxin and activated forms of Cry1Ac. At least 150 larvae (F11) were used in each experiment. LC50, 50% lethal concentration. FL, fiducial limits.
↵b Resistance ratio of 50% lethal concentrations for the UNSEL and Cry1Ac-SEL populations to those for the ROTH population.
↵c Expressed in E. coli.
↵d Solubilized in 50 mM Na2CO3 and, where indicated, activated with 1 mg of trypsin per ml.