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Mycology

Competitive PCR for Quantitation of a Cytophaga-Flexibacter-Bacteroides Phylum Bacterium Associated with the Tuber borchii Vittad. Mycelium

Elena Barbieri, Giulia Riccioni, Anna Pisano, Davide Sisti, Sabrina Zeppa, Deborah Agostini, Vilberto Stocchi
Elena Barbieri
1“Giorgio Fornaini” Institute of Biochemistry
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Giulia Riccioni
1“Giorgio Fornaini” Institute of Biochemistry
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Anna Pisano
1“Giorgio Fornaini” Institute of Biochemistry
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Davide Sisti
2Institute of Botany, University of Urbino, 61029 Urbino, Pesaro-Urbino, Italy
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Sabrina Zeppa
1“Giorgio Fornaini” Institute of Biochemistry
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Deborah Agostini
1“Giorgio Fornaini” Institute of Biochemistry
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Vilberto Stocchi
1“Giorgio Fornaini” Institute of Biochemistry
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  • For correspondence: v.stocchi@uniurb.it
DOI: 10.1128/AEM.68.12.6421-6424.2002
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Article Information

DOI 
https://doi.org/10.1128/AEM.68.12.6421-6424.2002
PubMed 
12450871

Published By 
American Society for Microbiology Journals
History 
  • Received April 12, 2002
  • Accepted August 29, 2002
  • Published online December 1, 2002.

Copyright & Usage 
Copyright © 2002 American Society for Microbiology

Author Information

  1. Elena Barbieri1,
  2. Giulia Riccioni1,
  3. Anna Pisano1,
  4. Davide Sisti2,
  5. Sabrina Zeppa1,
  6. Deborah Agostini1 and
  7. Vilberto Stocchi1*
  1. 1“Giorgio Fornaini” Institute of Biochemistry
  2. 2Institute of Botany, University of Urbino, 61029 Urbino, Pesaro-Urbino, Italy
  • ↵* Corresponding author. Mailing address: Istituto di Chimica Biologica “Giorgio Fornaini,” University of Urbino, Via A. Saffi, 2, 61029 Urbino (PU), Italy. Phone: 39 0722 305262. Fax: 39 0722 320188. E-mail: v.stocchi{at}uniurb.it .

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Competitive PCR for Quantitation of a Cytophaga-Flexibacter-Bacteroides Phylum Bacterium Associated with the Tuber borchii Vittad. Mycelium
Elena Barbieri, Giulia Riccioni, Anna Pisano, Davide Sisti, Sabrina Zeppa, Deborah Agostini, Vilberto Stocchi
Applied and Environmental Microbiology Dec 2002, 68 (12) 6421-6424; DOI: 10.1128/AEM.68.12.6421-6424.2002

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Competitive PCR for Quantitation of a Cytophaga-Flexibacter-Bacteroides Phylum Bacterium Associated with the Tuber borchii Vittad. Mycelium
Elena Barbieri, Giulia Riccioni, Anna Pisano, Davide Sisti, Sabrina Zeppa, Deborah Agostini, Vilberto Stocchi
Applied and Environmental Microbiology Dec 2002, 68 (12) 6421-6424; DOI: 10.1128/AEM.68.12.6421-6424.2002
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  • Top
  • Article
    • ABSTRACT
    • Mycelia and bacterial strain.
    • Ergosterol assay.
    • Construction of the QPCR competitor.
    • rRNA operon copy number determination for S. heparinum.
    • QPCR competitor.
    • Copies of the CFB bacterium 16S rDNA in T. borchii mycelium.
    • rRNA operon copy number determination for S. heparinum.
    • QPCR of the 16S rDNA in T. borchii mycelium strains.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Ascomycota
Bacteroides
Cytophaga
Flavobacterium
Mycelium
Polymerase Chain Reaction

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