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Applied and Environmental Microbiology
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Genetics and Molecular Biology

Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector

Franck Biet, Yves Cenatiempo, Christophe Fremaux
Franck Biet
1Institut de Biologie Moléculaire et d'Ingénierie Génétique, Equipe d’Accueil 2224, Université de Poitiers, 86022 Poitiers Cedex
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  • For correspondence: franck.biet@pasteur-lille.fr
Yves Cenatiempo
1Institut de Biologie Moléculaire et d'Ingénierie Génétique, Equipe d’Accueil 2224, Université de Poitiers, 86022 Poitiers Cedex
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Christophe Fremaux
2Rhodia Food, Zone d’Activités de Buxières, 86220 Dangé Saint-Romain, France
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DOI: 10.1128/AEM.68.12.6451-6456.2002
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ABSTRACT

A 2,665-bp cryptic plasmid, pTXL1, isolated from Leuconostoc mesenteroides subsp. mesenteroides Y110 was identified. This plasmid harbors a replicon localized on a 1,300-bp fragment. Two observations suggested that pTXL1 does not belong to rolling-circle replication (RCR)-type plasmids and most likely replicates via a theta mechanism. These hypotheses are supported by the observation that no detectable single-stranded intermediate was found for the replicon and that, unlike in RCR-type plasmids, the pTXL1 replicon sequence lacks an open reading frame encoding a replicase. The small-sized pTXL1 plasmid is stable and, according to its origin, can be considered in the “generally recognized as safe” category. Its ability to replicate in several lactic acid bacteria was exploited to develop a vector producing mesentericin Y105, a class II anti-Listeria bacteriocin. With this new vector, a recombinant industrial Leuconostoc cremoris strain able to produce mesentericin Y105 was constructed.

  • Copyright © 2002 American Society for Microbiology
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Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector
Franck Biet, Yves Cenatiempo, Christophe Fremaux
Applied and Environmental Microbiology Dec 2002, 68 (12) 6451-6456; DOI: 10.1128/AEM.68.12.6451-6456.2002

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Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector
Franck Biet, Yves Cenatiempo, Christophe Fremaux
Applied and Environmental Microbiology Dec 2002, 68 (12) 6451-6456; DOI: 10.1128/AEM.68.12.6451-6456.2002
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KEYWORDS

Genetic Vectors
Leuconostoc
plasmids
Replicon

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