Article Figures & Data
Figures
- FIG. 1.
DNA and deduced amino acid sequences of the Ent1071 structural genes and a gene involved in immunity to Ent1071 from E. faecalis FAIR-E 309. The presumptive −10 and −35 promoter sequences are indicated by boldface type, and RBSs are underlined. The direction of transcription of the genes is indicated by arrows. Triangles indicate the processing site for removal of the leader peptides of bacteriocin preproteins. The inverted repeats downstream of ent1071B and eni1071, which might act as rho-independent transcriptional terminators, are indicated by reversed arrows.
- FIG. 2.
(a) Alignment of the Ent1071A peptide sequences determined by Balla et al. (2) and in this study with the lactococcin Gα peptide sequence; (b) alignment of the Ent1071B peptide sequence determined by Balla et al. (2) and in this study with the lactococcin Gβ peptide sequence determined by Lipman-Pearson alignment. Identical amino acids are shown; similar amino acids are indicated by colons.
- FIG. 3.
DNA and deduced amino acid sequences of the 3′ end region of the Ent1071A gene and the 5′ start region of the Ent1071B gene for the Ent1071 locus as reported by Balla et al. (2) (a) and in this study (b). The deduced amino acid sequences following the two presumptive ATG start codons are shown for the Ent1071B gene, as determined in this study (b).
Tables
- TABLE 1.
Bacterial strains and plasmids used in this study
Bacterial strain or plasmid Relevant characteristicsa Reference(s) or source Strains E. faecalis FAIR-E 309 ent1071A + ent1071B + eni1071 + 19 E. faecalis BFE 1071 ent1071A + ent1071B + eni1071 + 2 E. faecalis ATCC 19433 Ent1071s, plasmidless 2; this study E. coli DH5α F−endA1 hsdR17 (rK−mK+) supE44 thi-1 λ−recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 Φ80 dlacZΔM15 BRL Life Technologies, Inc. E. coli MH1 MC1061 derivative, araD139 lacX74 galU galK hsr hsm+strA 4 Plasmids pUC118 lacZ′ Apr, 3.2 kb 21 pMG36e Expression vector, Emr, 3.6 kb 20 pBFE01 pUC118 containing 424-bp PCR product insert of ent1071A and ent1071B This study pBFE02 pUC118 containing 3.2-kb XbaI fragment containing Ent1071 genes This study pBFE03 pMG36e containing 407-bp PCR product insert of eni1071 This study ↵ a Apr, ampicillin resistant; Emr, erythromycin resistant.
