Article Figures & Data
Figures
- FIG. 1.
Silver-stained SDS-PAGE gel of LPS from S. algae BrYFC (lane 1), S. algae 136-2 (lane 2), S. algae BCM-8 (lane 3), S. amazonensis SB2B (lane 4), S. pealeana (lane 5), and S. woodyi (lane 6) cultured at 26°C.
- FIG. 2.
(A) Silver-stained SDS-PAGE gel of LPS from S. oneidensis MR-1 cells cultured at 5oC (lane 1), 15oC (lane 2), 20oC (lane 3), 26oC (lane 4), and 30°C (lane 5); (B) gel of LPS from S. frigidimarina cells cultured at 5oC (lane 1), 15oC (lane 2), 22oC (lane 3), and 26°C (lane 4).
- FIG. 3.
All are thin sections and the bar in each image is 100 nm. (A) Conventionally embedded S. oneidensis MR-1; OM, outer membrane; P, periplasm; PM, plasma membrane. Note the absence of fibrous material on the outer membrane of this cell. (B) Freeze-substitution of S. oneidensis MR-1 (a patch of fringe on the outer membrane is shown by the arrow, whereas adjacent regions do not have the fringe). (C) Freeze-substitution of S. algae BrYFC (only one of the two cells has a fringe). (D) Freeze-substitution of Shewanella sp. strain MR-4 (arrows indicate the ordered fibrous material external to the outer membrane). (E) Freeze-substitution of S. putrefaciens CN32 (no fringe is seen).
Tables
- TABLE 1.
Shewanella strains used in this study
Strain Collection no. Characteristics S. putrefaciens CN32 ATCC BAA-453 Commonly used Fe oxide-reducing strain S. putrefaciens NCTC 10695 Reference strain for Owen's homology group I S. oneidensis MR-1 ATCC 700550 Type strain S. oneidensis DLM-7 S. algae OK-1 ATCC 51192 Type strain S. algae 136-2 NCTC 10738 Reference strain for Owen's homology group IV S. algae BrY ATCC 51181 Commonly used Fe oxide-reducing strain S. algae BCM-8 Shewanella sp. strain MR-4 Shewanella sp. strain CL 256/73 NCTC 12093 Reference strain for Owen's homology group III S. baltica 63 NCTC 10735 Type strain S. amazonensis SB2B ATCC 700329 Type strain S. frigidimarina ACAM 591 Type strain S. pealeana ANG-SQ1 ATCC 700345 Type strain S. woodyi MS32 ATCC 51908 Type strain - TABLE 2.
Surface properties of used Shewanella strains
Strain Presence of S-LPS/PSa Presence of fringe (freeze substitution) S. putrefaciens CN32 − − S. putrefaciens NCTC 10695 − − S. baltica 63 − − Shewanella sp. strain CL 256/73 − NDb Shewanella sp. strain MR-4 − + S. oneidensis MR-1 − + S. oneidensis DLM-7 − + S. amazonensis SB2B + − S. algae OK-1 + + S. algae BrYDL + ±c S. algae BrYFC − ± S. algae 136-2 + ND S. algae BCM-8 + ND S. frigidimarina ACAM 591 + + S. pealeana ANG-SQ1 + ND S. woodyi MS32 + ND
