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Microbial Ecology

Microbial Characterization of Biofilms in Domestic Drains and the Establishment of Stable Biofilm Microcosms

Andrew J. McBain, Robert G. Bartolo, Carl E. Catrenich, Duane Charbonneau, Ruth G. Ledder, Alexander H. Rickard, Sharon A. Symmons, Peter Gilbert
Andrew J. McBain
1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, United Kingdom
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Robert G. Bartolo
2Procter and Gamble, Cincinnati, Ohio
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Carl E. Catrenich
2Procter and Gamble, Cincinnati, Ohio
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Duane Charbonneau
2Procter and Gamble, Cincinnati, Ohio
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Ruth G. Ledder
1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, United Kingdom
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Alexander H. Rickard
1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, United Kingdom
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Sharon A. Symmons
1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, United Kingdom
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Peter Gilbert
1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, United Kingdom
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  • For correspondence: peter.gilbert@man.ac.uk
DOI: 10.1128/AEM.69.1.177-185.2003
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Article Information

DOI 
https://doi.org/10.1128/AEM.69.1.177-185.2003
PubMed 
12513993

Published By 
American Society for Microbiology Journals
History 
  • Received June 13, 2002
  • Accepted October 6, 2002
  • Published online January 1, 2003.

Copyright & Usage 
Copyright © 2003 American Society for Microbiology

Author Information

  1. Andrew J. McBain1,
  2. Robert G. Bartolo2,
  3. Carl E. Catrenich2,
  4. Duane Charbonneau2,
  5. Ruth G. Ledder1,
  6. Alexander H. Rickard1,
  7. Sharon A. Symmons1 and
  8. Peter Gilbert1*
  1. 1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, United Kingdom
  2. 2Procter and Gamble, Cincinnati, Ohio
  • ↵* Corresponding author. Mailing address: School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, United Kingdom. Phone: 44 (0)161 275 2361. Fax: 44 (0)161 275 2396. E-mail: peter.gilbert{at}man.ac.uk .

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Microbial Characterization of Biofilms in Domestic Drains and the Establishment of Stable Biofilm Microcosms
Andrew J. McBain, Robert G. Bartolo, Carl E. Catrenich, Duane Charbonneau, Ruth G. Ledder, Alexander H. Rickard, Sharon A. Symmons, Peter Gilbert
Applied and Environmental Microbiology Jan 2003, 69 (1) 177-185; DOI: 10.1128/AEM.69.1.177-185.2003

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Microbial Characterization of Biofilms in Domestic Drains and the Establishment of Stable Biofilm Microcosms
Andrew J. McBain, Robert G. Bartolo, Carl E. Catrenich, Duane Charbonneau, Ruth G. Ledder, Alexander H. Rickard, Sharon A. Symmons, Peter Gilbert
Applied and Environmental Microbiology Jan 2003, 69 (1) 177-185; DOI: 10.1128/AEM.69.1.177-185.2003
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  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • Domestic drain microcosms.
    • Direct bacterial-cell counts.
    • Bacterial characterization by culture.
    • Community DNA extraction.
    • PCR amplification for DGGE analysis.
    • DGGE analysis.
    • Partial 16S rDNA gene sequencing of bacterial isolates and excised gel bands.
    • Identification of strains by comparative methods.
    • Construction of neighbor-joining tree.
    • Microscopic enumeration of metazoa.
    • MPN estimation of protozoa.
    • Identification of protozoa and metazoa.
    • Chemicals.
    • RESULTS AND DISCUSSION
    • Bacterial analysis.
    • Culture-independent analysis.
    • Stability of microcosms.
    • Phylogenetic characterization of biofilms by culture and DGGE.
    • Protozoa and metazoa.
    • Nucleotide sequence accession numbers.
    • Conclusions
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

bacteria
biofilms
Ecosystem
Water Supply

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