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Public Health Microbiology

Comparison of UV Inactivation of Spores of Three Encephalitozoon Species with That of Spores of Two DNA Repair-Deficient Bacillus subtilis Biodosimetry Strains

Marilyn M. Marshall, Samuel Hayes, Jackie Moffett, Charles R. Sterling, Wayne L. Nicholson
Marilyn M. Marshall
1Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721
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  • For correspondence: marshalm@u.arizona.edu
Samuel Hayes
2U.S. Environmental Protection Agency, Cincinnati, Ohio 45268
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Jackie Moffett
1Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721
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Charles R. Sterling
1Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721
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Wayne L. Nicholson
1Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721
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DOI: 10.1128/AEM.69.1.683-685.2003
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    FIG. 1.

    Summary of spore inactivation by 254-nm UV. B. subtilis biodosimetry strains WN333 (circles) and WN626 (triangles) at EPA (open symbols) or at UA (filled symbols). For comparison, the UV inactivation kinetics from Table 1 are plotted for spores of E. intestinalis (solid squares), E. cuniculi (hatched squares), and E. hellem (open squares). The data points shown for WN333 at UA are averages of three separate determinations, which varied by ±5%.

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  • TABLE 1.

    UV inactivation kinetics of E. intestinalis, E. hellem, and E. cuniculi sporesa

    SporesSiteUV fluence (J/m2)No. of infected/ no. inoculated (P)Response logitLive spores (D)Log applied dose (Do)Log change (D/Do)Temp (°C)
    E. intestinalis UA50141/5 (0.93)2.639057753.2−1.325
    UA601/10 (0.10)−2.19722513.2−3.225
    EPA602/15 (0.13)−1.871813.2−3.225
    UA803/15 (0.20)−1.38629413.2−3.225
    UA900/10 (0)3.2−3.225
    EPA900/10 (0)3.2−3.225
    UA1200/5 (0)3.2−3.225
    UA1400/5 (0)3.2−3.225
    UA1400/5 (0)3.2−3.25
    E. hellem UA1208/10 (0.80)1.386294553.2−1.525
    UA1405/10 (0.50)0163.2−2.025
    UA1702/10 (0.20)−1.38629443.2−2.625
    UA1900/10 (0)3.2−3.225
    E. cuniculi UA806/10 (0.60)0.405465293.2−1.825
    UA1004/10 (0.40)−0.40546583.2−2.325
    UA1302/10 (0.20)−1.38629423.2−3.025
    UA1400/5 (0)3.2−3.225
    • ↵ a Calculations performed according to Korich et al. (8). See the text for details.

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Comparison of UV Inactivation of Spores of Three Encephalitozoon Species with That of Spores of Two DNA Repair-Deficient Bacillus subtilis Biodosimetry Strains
Marilyn M. Marshall, Samuel Hayes, Jackie Moffett, Charles R. Sterling, Wayne L. Nicholson
Applied and Environmental Microbiology Jan 2003, 69 (1) 683-685; DOI: 10.1128/AEM.69.1.683-685.2003

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Comparison of UV Inactivation of Spores of Three Encephalitozoon Species with That of Spores of Two DNA Repair-Deficient Bacillus subtilis Biodosimetry Strains
Marilyn M. Marshall, Samuel Hayes, Jackie Moffett, Charles R. Sterling, Wayne L. Nicholson
Applied and Environmental Microbiology Jan 2003, 69 (1) 683-685; DOI: 10.1128/AEM.69.1.683-685.2003
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KEYWORDS

Bacillus subtilis
DNA repair
Encephalitozoon
Ultraviolet Rays

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