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Microbial Ecology

Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation

Jeremy P. Burton, Peter A. Cadieux, Gregor Reid
Jeremy P. Burton
1Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute
2Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
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  • For correspondence: jburton@lri.sjhc.london.on.ca
Peter A. Cadieux
2Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
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Gregor Reid
1Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute
2Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
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DOI: 10.1128/AEM.69.1.97-101.2003
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  • FIG. 1.
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    FIG. 1.

    DGGE of the V2-V3 16S rRNA gene amplicons from vaginal samples: profiles for 19 subjects (zero time, prestudy samples). The arrowheads indicate the DNA fragments sequenced from specific lanes, while in unmarked lanes only the dominant fragment was sequenced. BLAST sequence homologies are shown in Table 1.

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    FIG. 2.

    DGGE profiles of the vaginal microbiota from five women during the study. Lanes L contained known isolates L. fermentum RC-14 (a) and L. rhamnosus GR-1 (b). Lanes 1 to 5 contained amplicons from samples taken at zero time (prestudy) and at 3, 7, 14, and 21 days after instillation of capsules containing lactobacilli, respectively. The arrowheads indicate DNA fragments that were sequenced. Presumptive identities based on closest BLAST homologies are as follows: for subject 263, lane 1, L. delbrueckii; for subject 265, lane 1, L. crispatus; for subject 265, lane 3, Pseudomonas sp.; for subject 260, lane 1, L. iners; for subject 260, lane 2, L. crispatus; for subject 261, lane 1 (from top to bottom), L. iners, Arthrobacter sp., and G. vaginalis; for subject 261, lane 2, Pseudomonas sp.; for subject 261, lane 3, S. agalactiae; for subject 261, lane 5, L. iners (all fragments); for subject 268, lane 1, B. fibrisolvens and G. vaginalis (fragments 1 and 2, respectively).

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  • TABLE 1.

    BLAST analysis of vaginal bacterial V2-V3 16S rRNA sequences of excised fragments from DGGE gels (zero time)

    SubjectFragment in gelMost closely related bacterial sequence% IdentityAccession no.
    2501 Gardnerella vaginalis 98 M58744
    2521 Lactobacillus crispatus 100 AF257097
    2 Gardnerella vaginalis 98 M58744
    2531 Lactobacillus crispatus 98 AF257097
    2541 Lactobacillus iners 100 Y16329
    2551 Lactobacillus crispatus 97 AF257097
    2561 Lactobacillus crispatus 100 AF257097
    2 Lactobacillus iners 99 Y16329
    2571 Lactobacillus crispatus 98 AF257097
    2 Lactobacillus iners 100 Y16329
    2581 Streptococcus agalactiae 100 AF015927
    2591 Lactobacillus gasseri 100 AF243165
    2601 Lactobacillus iners 100 Y16329
    2611 Lactobacillus iners 99 Y16329
    2 Arthrobacter sp.100 AJ243423
    3 Gardnerella vaginalis 99 M58744
    2621 Lactobacillus acidophilus 97 AF375937
    2 Lactobacillus iners 96 Y16329
    2631 Lactobacillus delbrueckii 97 AF375917
    2641 Lactobacillus iners 92 Y16329
    2 Gardnerella vaginalis 98 M58744
    2651 Lactobacillus crispatus 98 AF257097
    2661 Lactobacillus iners 96 Y16329
    2671 Caulobacter sp.98 M83799
    2 Gardnerella vaginalis 97 M58744
    2681 Butyrivibrio fibrisolvens 95 AF125217
    2 Gardnerella vaginalis 97 M58744
    2691 Lactobacillus crispatus 99 AF257097
  • TABLE 2.

    Detection of Lactobacillus strains by selective culturing and subsequent RAPD analysis in a group of 10 women

    Lactobacillus strainNo. of women positive on the following days after instillation:
    371421
    GR-110862
    RC-145420
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Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation
Jeremy P. Burton, Peter A. Cadieux, Gregor Reid
Applied and Environmental Microbiology Jan 2003, 69 (1) 97-101; DOI: 10.1128/AEM.69.1.97-101.2003

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Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation
Jeremy P. Burton, Peter A. Cadieux, Gregor Reid
Applied and Environmental Microbiology Jan 2003, 69 (1) 97-101; DOI: 10.1128/AEM.69.1.97-101.2003
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KEYWORDS

bacteria
lactobacillus
Premenopause
probiotics
Vagina

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