Article Figures & Data
Figures
- FIG. 1.
DGGE of the V2-V3 16S rRNA gene amplicons from vaginal samples: profiles for 19 subjects (zero time, prestudy samples). The arrowheads indicate the DNA fragments sequenced from specific lanes, while in unmarked lanes only the dominant fragment was sequenced. BLAST sequence homologies are shown in Table 1.
- FIG. 2.
DGGE profiles of the vaginal microbiota from five women during the study. Lanes L contained known isolates L. fermentum RC-14 (a) and L. rhamnosus GR-1 (b). Lanes 1 to 5 contained amplicons from samples taken at zero time (prestudy) and at 3, 7, 14, and 21 days after instillation of capsules containing lactobacilli, respectively. The arrowheads indicate DNA fragments that were sequenced. Presumptive identities based on closest BLAST homologies are as follows: for subject 263, lane 1, L. delbrueckii; for subject 265, lane 1, L. crispatus; for subject 265, lane 3, Pseudomonas sp.; for subject 260, lane 1, L. iners; for subject 260, lane 2, L. crispatus; for subject 261, lane 1 (from top to bottom), L. iners, Arthrobacter sp., and G. vaginalis; for subject 261, lane 2, Pseudomonas sp.; for subject 261, lane 3, S. agalactiae; for subject 261, lane 5, L. iners (all fragments); for subject 268, lane 1, B. fibrisolvens and G. vaginalis (fragments 1 and 2, respectively).
Tables
- TABLE 1.
BLAST analysis of vaginal bacterial V2-V3 16S rRNA sequences of excised fragments from DGGE gels (zero time)
Subject Fragment in gel Most closely related bacterial sequence % Identity Accession no. 250 1 Gardnerella vaginalis 98 M58744 252 1 Lactobacillus crispatus 100 AF257097 2 Gardnerella vaginalis 98 M58744 253 1 Lactobacillus crispatus 98 AF257097 254 1 Lactobacillus iners 100 Y16329 255 1 Lactobacillus crispatus 97 AF257097 256 1 Lactobacillus crispatus 100 AF257097 2 Lactobacillus iners 99 Y16329 257 1 Lactobacillus crispatus 98 AF257097 2 Lactobacillus iners 100 Y16329 258 1 Streptococcus agalactiae 100 AF015927 259 1 Lactobacillus gasseri 100 AF243165 260 1 Lactobacillus iners 100 Y16329 261 1 Lactobacillus iners 99 Y16329 2 Arthrobacter sp. 100 AJ243423 3 Gardnerella vaginalis 99 M58744 262 1 Lactobacillus acidophilus 97 AF375937 2 Lactobacillus iners 96 Y16329 263 1 Lactobacillus delbrueckii 97 AF375917 264 1 Lactobacillus iners 92 Y16329 2 Gardnerella vaginalis 98 M58744 265 1 Lactobacillus crispatus 98 AF257097 266 1 Lactobacillus iners 96 Y16329 267 1 Caulobacter sp. 98 M83799 2 Gardnerella vaginalis 97 M58744 268 1 Butyrivibrio fibrisolvens 95 AF125217 2 Gardnerella vaginalis 97 M58744 269 1 Lactobacillus crispatus 99 AF257097 - TABLE 2.
Detection of Lactobacillus strains by selective culturing and subsequent RAPD analysis in a group of 10 women
Lactobacillus strain No. of women positive on the following days after instillation: 3 7 14 21 GR-1 10 8 6 2 RC-14 5 4 2 0
