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Enzymology and Protein Engineering

Family Shuffling of Expandase Genes To Enhance Substrate Specificity for Penicillin G

Jyh-Shing Hsu, Yunn-Bor Yang, Chan-Hui Deng, Chia-Li Wei, Shwu-Huey Liaw, Ying-Chieh Tsai
Jyh-Shing Hsu
1Institute of Biochemistry
2Synmax Biochemical Co., Ltd., Hsinchu, Taiwan
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Yunn-Bor Yang
2Synmax Biochemical Co., Ltd., Hsinchu, Taiwan
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Chan-Hui Deng
2Synmax Biochemical Co., Ltd., Hsinchu, Taiwan
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Chia-Li Wei
1Institute of Biochemistry
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Shwu-Huey Liaw
1Institute of Biochemistry
3and Faculty of Life Science, National Yang-Ming University, Taipei
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  • For correspondence: shliaw@ym.edu.tw tsaiyc@ym.edu.tw
Ying-Chieh Tsai
1Institute of Biochemistry
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  • For correspondence: shliaw@ym.edu.tw tsaiyc@ym.edu.tw
DOI: 10.1128/AEM.70.10.6257-6263.2004
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Article Information

DOI 
https://doi.org/10.1128/AEM.70.10.6257-6263.2004
PubMed 
15466573

Published By 
American Society for Microbiology Journals
History 
  • Received February 25, 2004
  • Accepted June 16, 2004
  • Published online October 4, 2004.

Copyright & Usage 
Copyright © 2004 American Society for Microbiology

Author Information

  1. Jyh-Shing Hsu1,2,
  2. Yunn-Bor Yang2,
  3. Chan-Hui Deng2,
  4. Chia-Li Wei1,
  5. Shwu-Huey Liaw1,3* and
  6. Ying-Chieh Tsai1*
  1. 1Institute of Biochemistry
  2. 3and Faculty of Life Science, National Yang-Ming University, Taipei
  3. 2Synmax Biochemical Co., Ltd., Hsinchu, Taiwan
  • ↵* Corresponding author. Mailing address: Institute of Biochemistry, National Yang-Ming University, 155 Li-Nong St., Sec. 2, Pei-Tou, Taipei 11221, Taiwan. Phone: (886) 2-2826-7125. Fax: (886) 2-2826-4843. E-mail for S.-H. Liaw: shliaw{at}ym.edu.tw . E-mail for Y.-C. Tsai: tsaiyc{at}ym.edu.tw .

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Family Shuffling of Expandase Genes To Enhance Substrate Specificity for Penicillin G
Jyh-Shing Hsu, Yunn-Bor Yang, Chan-Hui Deng, Chia-Li Wei, Shwu-Huey Liaw, Ying-Chieh Tsai
Applied and Environmental Microbiology Oct 2004, 70 (10) 6257-6263; DOI: 10.1128/AEM.70.10.6257-6263.2004

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Family Shuffling of Expandase Genes To Enhance Substrate Specificity for Penicillin G
Jyh-Shing Hsu, Yunn-Bor Yang, Chan-Hui Deng, Chia-Li Wei, Shwu-Huey Liaw, Ying-Chieh Tsai
Applied and Environmental Microbiology Oct 2004, 70 (10) 6257-6263; DOI: 10.1128/AEM.70.10.6257-6263.2004
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KEYWORDS

Genes, Bacterial
Intramolecular Transferases
Penicillin G
penicillin-binding proteins
Streptomyces

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