Role of Hippoboscidae Flies as Potential Vectors of Bartonella spp. Infecting Wild and Domestic Ruminants

Lénaïg Halos; Taoufik Jamal; Renaud Maillard; Benjamin Girard; Jacques Guillot; Bruno Chomel; Muriel Vayssier-Taussat; Henri-Jean Boulouis

doi:10.1128/AEM.70.10.6302-6305.2004

DOI: 10.1128/AEM.70.10.6302-6305.2004

Article Figures & Data

Tables

TABLE 1.

Taxonomic identification, origin, and repartition of the 83 samples of Hippoboscidae tested, and results of specific PCR amplification of their Bartonella citrate synthase (gltA) genes

Species of Hippoboscidae	Evolutive stage	No. of flies	Host (no. of hosts)	Origin (yr of sampling)	No. of samples positive by PCR for Bartonella gltA gene/ total no. of samples (%)
Lipoptena cervi	Adult	10	Wild roe deer (1)	Midi Pyrénéces (2000)	8/10	= 45/48 (94)
		14	Wild roe deer (unknown)	Unknown (1994)	13/14
		24	Wild roe deer (1)	Bourgogne (2003)	24/14
	Pupa	3	Wild roe deer (unknown)	Unknown (1994)	0/3	= 0/3 (0)
Hippobosca equina	Adult	2	Cow (1)	Ile de France (2001)	2/2	= 12/12 (100)
		10	Cow (3)	Rhone Alpes (2002)	10/10	= 12/12 (100)
	Adult	5	Horse (1)	Centre (2003)	0/5	= 0/5 (0)
	Pupa	1	Horse (1)	Centre (2003)	0/1	= 0/1 (0)
Melophagus ovinus	Adult	6	Sheep (1)	Normandie (2003)	6/6	= 20/20 (100)
		4	Sheep (1)	Ile de France (2002)	1/1
		2	Sheep (1)	Bourgogne (2003)	1/1
		8	Sheep (2)	Roumanie (2004)	8/8
	Pupa	3	Sheep (unknown)	Unknown	3/3	= 10/10 (100)
		2	Sheep (2)	Normandie (2003)	2/2
		1	Sheep (1)	Bourgogne (2003)	1/1
		4	Sheep (2)	Roumanie (2004)	4/4

TABLE 2.

PCR-RFLP profiles, distribution, and sequence identification by partial sequence analysis of the gltA genes of ruminant-infecting Bartonella-reference strains obtained from different species of Hippoboscidae

PCR-RFLP profile type (associated strain[s])	Fragment size(s) (bp) after digestion with^a:			Species of Hippoboscidae flies showing the profile type	No. of samples	No. of sequenced fragments	Closest Bartonella species (% DNA identity based on a fragment of the gltA gene)
PCR-RFLP profile type (associated strain[s])	TaqI	DdeI	MseI	Species of Hippoboscidae flies showing the profile type	No. of samples	No. of sequenced fragments
1 (B. schoenbuchensis R1)	ND	300, U	180, U	L. cervi	26	4	B. schoenbuchensis R1 (100)
				M. ovinus			No sequence available
				Adult	1
				Pupa	3
2 (B. chomelii, B. schoenbuchensis	ND	ND	180, U	L. cervi	4	1	B. schoenbuchensis R3 (100)
R3, and A971)				H. equina	12	1	B. chomelii (100)
				M. ovinus			A971 cervid strain (99)
				Adult	1	1
				Pupa	1	1
3 (B. bovis)	150, 250	ND	150, 220	None
4 (B. capreoli)	ND	100, 250	120, 180	None
New (no strain associated)	ND	ND	200, 180	L. cervi	6	2	A971 cervid strain (95)
Mixed (profiles 1 and 2)	ND	ND, 300, U	180, U	L. cervi	7	1
						Clone 1	B. schoenbuchensis R1 (100)
						Clone 2	B. chomelii (100)