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Genetics and Molecular Biology

Dynamics of Multiple lin Gene Expression in Sphingomonas paucimobilis B90A in Response to Different Hexachlorocyclohexane Isomers

Mrutyunjay Suar, Jan Roelof van der Meer, Kirsten Lawlor, Christof Holliger, Rup Lal
Mrutyunjay Suar
1Department of Zoology, University of Delhi, Delhi, India
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Jan Roelof van der Meer
2Process of Environmental Microbiology and Molecular Ecotoxicology, Swiss Federal Institute for Environmental Science and Technology (EAWAG), Duebendorf
3Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland
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  • For correspondence: JanRoelof.VanDerMeer@unil.ch
Kirsten Lawlor
2Process of Environmental Microbiology and Molecular Ecotoxicology, Swiss Federal Institute for Environmental Science and Technology (EAWAG), Duebendorf
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Christof Holliger
4and Laboratory for Environmental Biotechnology, ENAC-ISTE, EPFL
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Rup Lal
1Department of Zoology, University of Delhi, Delhi, India
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DOI: 10.1128/AEM.70.11.6650-6656.2004
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ABSTRACT

Sphingomonas paucimobilis B90A is able to degrade the α-, β-, γ-, and δ-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown S. paucimobilis B90A by RNA dot blot hybridization and real-time reverse transcriptase PCR upon exposure to a pulse of different HCH isomers. Irrespective of the addition of HCH, linA, linB, and linC were all expressed constitutively. In contrast, linD and linE were induced with α-HCH (2 mg/liter) and γ-HCH (7 mg/liter). A sharp increase in mRNA levels for linD and linE was observed from 10 to 45 min after the addition of α- or γ-HCH. Induction of linD and linE was not detectable upon the addition of 0.7 mg of γ-HCH per liter, although the compound was degraded by the cells. The addition of β-HCH (5 mg/liter) or δ-HCH (20 mg/liter) did not lead to linE and linD induction, despite the fact that 50% of the compounds were degraded. This suggests that degradation of β- and δ-HCH proceeds by a different pathway than that of α- and γ-HCH.

  • Copyright © 2004 American Society for Microbiology
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Dynamics of Multiple lin Gene Expression in Sphingomonas paucimobilis B90A in Response to Different Hexachlorocyclohexane Isomers
Mrutyunjay Suar, Jan Roelof van der Meer, Kirsten Lawlor, Christof Holliger, Rup Lal
Applied and Environmental Microbiology Nov 2004, 70 (11) 6650-6656; DOI: 10.1128/AEM.70.11.6650-6656.2004

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Dynamics of Multiple lin Gene Expression in Sphingomonas paucimobilis B90A in Response to Different Hexachlorocyclohexane Isomers
Mrutyunjay Suar, Jan Roelof van der Meer, Kirsten Lawlor, Christof Holliger, Rup Lal
Applied and Environmental Microbiology Nov 2004, 70 (11) 6650-6656; DOI: 10.1128/AEM.70.11.6650-6656.2004
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KEYWORDS

Bacterial Proteins
Gene Expression Regulation, Bacterial
Hexachlorocyclohexane
Lyases
Sphingomonas

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