Article Figures & Data
Figures
- FIG. 1.
PCR-SSCP electropherograms representing the range of bulk soil to rhizosphere comparisons found in this study. Paired samples exhibiting major differences in relative diversity were found for all of the terrestrial plant groups sampled (A), and paired samples exhibiting very similar PCR-SSCP profiles were also found for all of the plant groups sampled (B). The x and y axes of each electropherogram represent relative migration distance and relative fluorescence intensity, respectively.
- FIG. 2.
PCR-SSCP electropherograms of bulk soil and rhizosphere samples collected at two sampling plots located by Stone's Pocket Road. Six paired samples were collected from sampling plot I (A), and seven paired samples were collected from sampling plot J (B). Plot I samples produced minor differences between the rhizosphere and the corresponding bulk soil. In contrast, plot J samples exhibited major differences. The x and y axes of each electropherogram represent relative migration distance and relative fluorescence intensity, respectively.
- FIG. 3.
PCA ordination of the difference between rhizosphere and bulk soil PCR-SSCP profiles (dmatrix) by sampling plot. Plot designations A to L are defined in Table 1. prin1 (17% variance explained) is plotted along the x axis, and prin2 (13% variance explained) is plotted along the y axis. The origin represents identical paired PCR-SSCP rhizosphere and bulk soil profiles (dmatrix = 0). Each data point represents a single paired rhizosphere and bulk soil sample (n = 76 across all plots).
Tables
- TABLE 1.
Rhizosphere sampling plots
Sampling plota Location Soil series (classification) Plant groups sampledc No. of samplesb UTMd (zone 16) coordinates Collection date A Hancock Plainfield sand (Typic Udipsamment) 2b, 3d, 1g 6 4,888,042.3 296,967.1 10/11/01 B Stone's Pocket Rd. Baraboo silt loam (Typic Hapludalf) 1p, 1d, 1m 3 4,807,494.2 273,380.4 10/19/01 C Simpson Rd. Marshan loam (Typic Haplaquoll) 1b, 1l, 1p, 1g, 1d, 1m 6 4,829,635.3 265,720.1 10/19/01 D Hancock Plainfield sand (Typic Udipsamment) 2g, 2d, 2m 6 4,888,134.2 296,995.3 11/15/01 E Hancock Plainfield sand (Typic Udipsamment) 2b, 2g, 2d, 2m 8 4,888,174.1 296,990.6 11/16/01 F Hancock Plainfield sand (Typic Udipsamment) 1b, 2g, 2d, 2m 7 4,888,167.1 297,023.5 11/16/01 G Picnic Point Kidder silt loam (Typic Hapludalf) 4d, 4m 8 4,773,268.2 302,360.0 11/16/01 H Picnic Point St. Charles silt loam (Typic Hapludalf) 2b, 3d 5 4,773,223.5 302,369.4 11/20/01 I Stone's Pocket Rd. Baraboo silt loam (Typic Hapludalf) 1b, 1l, 1p, 1g, 1d, 1m 6 4,807,494.3 273,378.4 11/21/01 J Stone's Pocket Rd. Baraboo silt loam (Typic Hapludalf) 1b, 2l, 1p, 1g, 1d, 1m 7 4,807,573.2 273,414.0 11/21/01 K Simpson Rd. Boone sands (Typic Quartzipsamment) 2l, 1p, 1g, 1d, 1m 6 4,830,035.3 265,767.1 11/26/01 L Stone's Pocket Rd. Baraboo stoney silt loam (Typic Hapludalf) 2b, 1l, 2p, 1g, 1d, 1m 8 4,807,479.2 273,395.4 11/28/01 ↵ a Nonoverlapping sampling plots were no larger than 50 m2.
↵ b Number of paired samples (rhizosphere and corresponding bulk soil). Total, 76.
↵ c b, bryophyte (moss); l, lycopod (club moss); p, pteridophyte (fern); g, gymnosperm (conifer); d, dicotyledonous (dicot); m, monocotyledonous (monocot).
↵ d UTM, Universal Transverse Mercator.
