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Microbial Ecology

Characterization of Nocardia asteroides Isolates from Different Ecological Habitats on the Basis of Repetitive Extragenic Palindromic-PCR Fingerprinting

Hideki Yamamura, Masayuki Hayakawa, Youji Nakagawa, Yuzuru Iimura
Hideki Yamamura
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu 400, Japan
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Masayuki Hayakawa
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu 400, Japan
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  • For correspondence: hayakawa@ab11.yamanashi.ac.jp
Youji Nakagawa
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu 400, Japan
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Yuzuru Iimura
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu 400, Japan
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DOI: 10.1128/AEM.70.5.3149-3151.2004
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    FIG. 1.

    Fingerprint patterns of Nocardia asteroides strains, obtained by REP-PCR with the BOX-A1R primer. Lanes: 1, 100-bp DNA ladder; 2, LS 001-3; 3, LS 001-4; 4, LS 002-1; 5, LS 002-2; 6, LS 002-5; 7, MS 002-1; 8, AS 004-5; 9, SO 031-3; 10, SO 008-1; 11, SO 008-2; 12, SO 020-4; 13, SO 026-3; 14, SO 027-1; 15, N. asteroides IFO 3384; 16, N. asteroides IFO 3424; 17, N. asteroides JCM 3384T; 18, 100-bp DNA ladder.

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    FIG. 2.

    Dendrogram of REP-PCR genomic fingerprints of N. asteroides strains. Percent similarity between patterns was calculated using Pearson coefficients. The clustering pattern was generated using the UPGMA method.

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  • TABLE 1.

    N. asteroides strains used in this study

    N. asteroides strainSource of isolate or strain descriptiona
    Environmental isolates
        SO 008-1, SO 008-2Vegetable field soil
        SO 020-4Paddy field soil
        SO 026-1Vegetable field soil
        SO 027-1Cornfield soil
        SO 031-3Flatland forest soil
        LS 001-3, LS 001-4Lake Suwa surface sediment (center area, 5 m depth)
        LS 002-1, LS 002-2, LS 002-5 Lake Suwa surface sediment (near the east     shore, 1 m depth)
        MS 002-1Takeda Shrine moat surface sediment (0.5 m depth)
        AS 004-5Scum of activated sludge
    N. asteroides JCM 3384TATCC 19247T; proposed working typeb
    N. asteroides IFO 3423FMJ 101; clinical isolate
    N. asteroides IFO 3424FMJ 102; clinical isolate
    • ↵ a ATCC, American Type Culture Collection, Rockville, Md.; FMJ, Faculty of Medicine, Juntendo University, Tokyo, Japan.

    • ↵ b See reference 17.

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Characterization of Nocardia asteroides Isolates from Different Ecological Habitats on the Basis of Repetitive Extragenic Palindromic-PCR Fingerprinting
Hideki Yamamura, Masayuki Hayakawa, Youji Nakagawa, Yuzuru Iimura
Applied and Environmental Microbiology May 2004, 70 (5) 3149-3151; DOI: 10.1128/AEM.70.5.3149-3151.2004

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Characterization of Nocardia asteroides Isolates from Different Ecological Habitats on the Basis of Repetitive Extragenic Palindromic-PCR Fingerprinting
Hideki Yamamura, Masayuki Hayakawa, Youji Nakagawa, Yuzuru Iimura
Applied and Environmental Microbiology May 2004, 70 (5) 3149-3151; DOI: 10.1128/AEM.70.5.3149-3151.2004
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  • Article
    • ABSTRACT
    • N. asteroides isolates.
    • REP-PCR.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

DNA Fingerprinting
Nocardia Infections
Nocardia asteroides
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
soil microbiology
Water Microbiology

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