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Public Health Microbiology

Methods for Enhanced Culture Recovery of Francisella tularensis

Jeannine M. Petersen, Martin E. Schriefer, Kenneth L. Gage, John A. Montenieri, Leon G. Carter, Miles Stanley, May C. Chu
Jeannine M. Petersen
Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
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  • For correspondence: nzp0@cdc.gov
Martin E. Schriefer
Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
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Kenneth L. Gage
Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
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John A. Montenieri
Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
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Leon G. Carter
Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
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Miles Stanley
Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
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May C. Chu
Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
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DOI: 10.1128/AEM.70.6.3733-3735.2004
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    FIG. 1.

    Comparison of carcass tissues plated on CHAB (left panel) and on CHAB-A (right panel).

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  • TABLE 1.

    Comparison of F. tularensis recovery rates for on-site culture and antibiotic-supplemented CHAB (CHAB-A)

    Prairie dogs (n)Culture recovery (%)a with:
    CHAB (on-site)CHAB-A
    Group A, live animals (20)18 (90)ND
    Group B, carcasses (46)9 (19.6)30 (81.1)b
    • ↵ a The difference in on-site recovery rates for group A versus group B and the difference in recovery rates for carcass tissues cultured on CHAB versus CHAB-A were statistically significant (P ≤ 0.05, Fisher's exact test). ND, not determined.

    • ↵ b Thirty-seven CHAB culture-negative prairie dogs were tested.

  • TABLE 2.

    Comparison of transport methods for culture recovery of F. tularensis from live and dead animalsa

    Prairie dogs (n)bCulture recovery (%) with:
    Cary-Blair mediaFrozen tissue
    Carcasses (9)1 (11.1)8 (88.8)
    Live animals (17)15 (88.2)16 (94.1)
    • ↵ a No statistically significant difference was observed when transport systems were compared for recovery of F. tularensis from live animals (P ≥ 0.05, McNemar's test). The difference in transport systems for dead animals was statistically significant (P ≤ 0.05, McNemar's test).

    • ↵ b Twenty-six animals from which F. tularensis was cultured by on-site inoculation of CHAB plates.

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Methods for Enhanced Culture Recovery of Francisella tularensis
Jeannine M. Petersen, Martin E. Schriefer, Kenneth L. Gage, John A. Montenieri, Leon G. Carter, Miles Stanley, May C. Chu
Applied and Environmental Microbiology Jun 2004, 70 (6) 3733-3735; DOI: 10.1128/AEM.70.6.3733-3735.2004

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Methods for Enhanced Culture Recovery of Francisella tularensis
Jeannine M. Petersen, Martin E. Schriefer, Kenneth L. Gage, John A. Montenieri, Leon G. Carter, Miles Stanley, May C. Chu
Applied and Environmental Microbiology Jun 2004, 70 (6) 3733-3735; DOI: 10.1128/AEM.70.6.3733-3735.2004
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KEYWORDS

Disease Outbreaks
Francisella tularensis
Rodent Diseases
Sciuridae
Specimen Handling
Tularemia

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