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Methods

Development of PCR Assays Targeting Genes in O-Antigen Gene Clusters for Detection and Identification of Escherichia coli O45 and O55 Serogroups

Chitrita DebRoy, Pina M. Fratamico, Elisabeth Roberts, Michael A. Davis, Yanhong Liu
Chitrita DebRoy
1Gastroenteric Disease Center, Department of Veterinary Science, The Pennsylvania State University, University Park, Pennsylvania 16802
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  • For correspondence: rcd3@psu.edu
Pina M. Fratamico
2United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038
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Elisabeth Roberts
1Gastroenteric Disease Center, Department of Veterinary Science, The Pennsylvania State University, University Park, Pennsylvania 16802
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Michael A. Davis
1Gastroenteric Disease Center, Department of Veterinary Science, The Pennsylvania State University, University Park, Pennsylvania 16802
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Yanhong Liu
2United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038
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DOI: 10.1128/AEM.71.8.4919-4924.2005
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Article Information

DOI 
https://doi.org/10.1128/AEM.71.8.4919-4924.2005
PubMed 
16085897

Published By 
American Society for Microbiology Journals
History 
  • Received October 11, 2004
  • Accepted February 21, 2005
  • Published online August 5, 2005.

Copyright & Usage 
Copyright © 2005 American Society for Microbiology

Author Information

  1. Chitrita DebRoy1*,
  2. Pina M. Fratamico2,
  3. Elisabeth Roberts1,
  4. Michael A. Davis1 and
  5. Yanhong Liu2
  1. 1Gastroenteric Disease Center, Department of Veterinary Science, The Pennsylvania State University, University Park, Pennsylvania 16802
  2. 2United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038
  • ↵* Corresponding author. Mailing address: Gastroenteric Disease Center, The Pennsylvania State University, Wiley Lab, Wiley Lane, University Park, PA 16802. Phone: (814) 863-2630. Fax: (814) 863-2167. E-mail: rcd3{at}psu.edu .

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Development of PCR Assays Targeting Genes in O-Antigen Gene Clusters for Detection and Identification of Escherichia coli O45 and O55 Serogroups
Chitrita DebRoy, Pina M. Fratamico, Elisabeth Roberts, Michael A. Davis, Yanhong Liu
Applied and Environmental Microbiology Aug 2005, 71 (8) 4919-4924; DOI: 10.1128/AEM.71.8.4919-4924.2005

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Development of PCR Assays Targeting Genes in O-Antigen Gene Clusters for Detection and Identification of Escherichia coli O45 and O55 Serogroups
Chitrita DebRoy, Pina M. Fratamico, Elisabeth Roberts, Michael A. Davis, Yanhong Liu
Applied and Environmental Microbiology Aug 2005, 71 (8) 4919-4924; DOI: 10.1128/AEM.71.8.4919-4924.2005
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KEYWORDS

Escherichia coli
Escherichia coli Proteins
Multigene Family
O Antigens
Polymerase Chain Reaction

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