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Applied and Environmental Microbiology
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Public Health Microbiology

Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces

C. Andrew Carson, Jessica M. Christiansen, Helen Yampara-Iquise, Verel W. Benson, Claire Baffaut, Jerri V. Davis, Robert R. Broz, William B. Kurtz, Wendi M. Rogers, William H. Fales
C. Andrew Carson
1Department of Veterinary Pathobiology
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  • For correspondence: carsonc@missouri.edu
Jessica M. Christiansen
1Department of Veterinary Pathobiology
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Helen Yampara-Iquise
1Department of Veterinary Pathobiology
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Verel W. Benson
2Department of Agricultural Economics—Food and Agricultural Policy Research Institute
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Claire Baffaut
2Department of Agricultural Economics—Food and Agricultural Policy Research Institute
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Jerri V. Davis
6U.S. Geological Survey, Rolla, Missouri 65401
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Robert R. Broz
3Agricultural Extension—Food Science and Nutrition
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William B. Kurtz
5Department of Forestry, University of Missouri, Columbia, Missouri 65211
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Wendi M. Rogers
2Department of Agricultural Economics—Food and Agricultural Policy Research Institute
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William H. Fales
4Veterinary Medical Diagnostic Laboratory
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DOI: 10.1128/AEM.71.8.4945-4949.2005
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ABSTRACT

A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron, generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples. The marker was not detected in 1 ng of fecal DNA from 61 cows, 35 horses, 44 pigs, 24 chickens, 29 turkeys, and 17 geese.

  • Copyright © 2005 American Society for Microbiology
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Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces
C. Andrew Carson, Jessica M. Christiansen, Helen Yampara-Iquise, Verel W. Benson, Claire Baffaut, Jerri V. Davis, Robert R. Broz, William B. Kurtz, Wendi M. Rogers, William H. Fales
Applied and Environmental Microbiology Aug 2005, 71 (8) 4945-4949; DOI: 10.1128/AEM.71.8.4945-4949.2005

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Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces
C. Andrew Carson, Jessica M. Christiansen, Helen Yampara-Iquise, Verel W. Benson, Claire Baffaut, Jerri V. Davis, Robert R. Broz, William B. Kurtz, Wendi M. Rogers, William H. Fales
Applied and Environmental Microbiology Aug 2005, 71 (8) 4945-4949; DOI: 10.1128/AEM.71.8.4945-4949.2005
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  • Top
  • Article
    • ABSTRACT
    • Preliminary validation of B. thetaiotaomicron marker.
    • Collection of human and nonhuman fecal samples.
    • Specificity of the B. thetaiotaomicron marker.
    • Sensitivity study.
    • Comparison to a benchmark human Bacteroides marker.
    • Application to environmental water samples.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

Bacteroides
DNA, Bacterial
feces
genetic markers
Water Pollution

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