Article Figures & Data
Figures
- FIG. 1.
Proportions of SSU sequences belonging to different fungal phyla and other eukaryotic kingdoms. The width of triangles corresponds to the number of sequences in each, and the height corresponds to the maximum amount of sequence divergence within clades (see scale bars).
- FIG. 2.
Proportional distribution of different taxonomic groups in ITS clone libraries. The x axis indicates the proportion of sequences assigned to each taxonomic group, and bar width corresponds to the proportion of all sequences belonging to each library. A. Proportion of all ITS sequences belonging to each fungal kingdom. B. Proportion of Ascomycota ITS sequences belonging to each subclass. C. Proportion of Basidiomycota ITS sequences belonging to each order.
- FIG. 3.
Species-effort curves of richness for fungal OTUs. A. Mixed hardwood plot. B. Pine plot. C. Pooled data from both plots.
- FIG. 4.
Species-effort curves for observed and estimated fungal OTU richness in each plot.
Tables
- TABLE 1.
Number of sequences and OTUs recovered from each library
Library Amt of template DNA (μg) No. of sequences No. of OTUs Ha No. of chimeras MH-L-1 30 43 18 3.98 2 MH-L-2b 240 101 67 5.60 0 MH-O-1 3 25 23 4.45 0 MH-O-2 3 50 39 5.15 0 MH-O-3 3 18 16 4.04 1 MH-O-4 150 72 53 5.57 3 MH-O-5c 3 29 25 4.56 0 MH-O-6c 3 23 16 3.58 0 MH-A-1b 240d 101 39 3.62 13 MH-A-2b 240d 87 62 5.60 0 MH-B-1b 240d 70 33 4.33 2 P-L-1b 240d 68 48 5.34 9 P-O-1b 240d 62 43 5.14 4 P-A-1 30 103 62 5.61 3 P-B-1 30 123 61 5.44 3 a Shannon-Weiner diversity index.
↵b Library generated from two pooled PCR products amplified using the modified “soil PCR” thermal cycler profile (see Materials and Methods).
↵c PCR primers ITS1F (MH-O-5 and -6) and ITS4B (MH-O-6 only) were used in place of ITS1 and ITS4.
↵d Template consisted of three pooled Mo Bio DNA preps.
- TABLE 2.
Number of sequences recovered for the most common genera encountereda
Genus (order/subclass) No. of sequences in library: MH-L P-L MH-O P-O MH-A P-A MH-B P-B Total Russula (Russulales) 0 0 4 2 2 12 2 13 35 Mycena (Agaricales) 11 2 0 0 0 0 0 3 16 Athelia (Polyporales) 1 2 4 1 0 0 0 1 9 Phialophora (Leotiomycetidae) 3 0 2 0 0 0 0 3 8 Tomentella (Thelephorales) 0 0 3 0 2 1 0 1 7 Hormonema (Dothidiomycetidae) 3 3 0 0 0 0 0 0 6 Alternaria (Dothidiomycetidae) 4 1 0 0 0 0 0 0 5 Cryptococcus (Tremellales) 0 2 1 0 0 0 1 1 5 Hygrocybe (Agaricales) 0 0 0 0 3 2 0 0 5 Tylospora (Polyporales) 0 0 4 0 0 0 0 1 5 ↵a Excluding MH-O-5, MH-O-6, and MH-A-1.
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 -
Identities of ITS OTUs (greater than or equal to 97% similar) and distributions across libraries (Table S1).
PDF document, 313K. - Supplemental file 2 -
Identities of ITS OTUs (100% similarity) and distributions across libraries (Table S2).
PDF document, 64K.
- Supplemental file 1 -
Identities of ITS OTUs (greater than or equal to 97% similar) and distributions across libraries (Table S1).
