Article Figures & Data
Figures
- FIG. 1.
Multiple sequence alignment of the P. furiosus AdhD with (hypothetical) members of the aldo-keto reductase superfamily. The following abbreviations are used: Pyrfu, P. furiosus; Theko, T. kodakaraensis; Pyrab, P. abyssi; Sulso, S. solfataricus; Thema, T. maritima; Suscr, S. scrofa. The sequences were aligned using the CLUSTAL program. Asterisks indicate highly conserved residues within the AKR superfamily.
- FIG. 2.
SDS-PAGE analysis of heterologously produced AdhD in each purification step. Lane 1, broad-host-range protein marker; lane 2, cell extract; lane 3, heat-stable cell extract; lane 4, pooled Q-Sepharose fractions. Molecular mass markers are given to the left of the gel.
- FIG. 3.
Specific activity of P. furiosus AdhD toward primary and secondary alcohols.
Tables
- TABLE 1.
Substrate specificity of P. furiosus AdhD in the oxidation reaction
Substratea Relative activity (%) 1-Pentanol 1 2-Pentanol 9 1,2-Propanediol 3 Glycerol 1 3-Phenyl-1-propanol 1 2-Methyl-1-propanol 2 2-Propen-1-ol 1 1-Butanol 1 2-Butanol 7 1,2-Butanediol 2 1,3-Butanediol 3 2,3-Butanediol 100 Acetoin 18 3-Phenyl-1-butanol 0.4 3-Methyl-1-butanol 0.4 d-Arabinose 66 l-Arabinose 0 d-Xylose 3 d-Glucose 2 Cyclohexanol 12 Benzyl alcohol 3 ↵ a Substrates were present in 100 mM concentrations.
- TABLE 2.
Substrate specificity of P. furiosus AdhD in the reduction reaction
Substratea Relative activity (%) Acetone 0.1 Dihydroxyacetone 36 Dihydroxyacetone phosphate 82 Hexanal 16 2-Hexanone 0.2 Decanal 1 Acetoin 100 Diacetyl ∼150b dl-Glyceraldehyde 31 Pyruvic aldehyde 81 d-Arabinose 25 d-Xylose 2 d-Glucose 0 d-Ribose 2 d-Mannose 2 Cyclohexanone 38
