Article Figures & Data
Figures
- FIG. 1.
Agarose gel electrophoresis of DNA fragments amplified by the ETEC/STEC multiplex PCR. Lanes: 1, 1-kb Plus DNA ladder (Invitrogen, Calrsbad, CA); 2, mixed DNA from strains 35150 and 35401; 3, MDP 04-02111; 4, FDA119. The products shown in lane 2 (from top to bottom) and their sizes are as follows: LT, 416 bp; stx, 313 bp; 16S rRNA gene, 206 bp; ST, 169 bp.
- FIG. 2.
PFGE profiles of XbaI-digested genomic DNA and the dendrogram comparing profile similarities. The cluster tree was constructed using BioNumerics (Applied Maths, Sint-Martens-Latem, Belgium) from comparisons of XbaI-digested PFGE patterns of the strains listed in Table 2. Opt, optimization; Tol, tolerance; H, height; S, surface.
Tables
- TABLE 1.
ETEC/STEC multiplex PCR primers used to amplify specific fragments from the LT, ST, Stx1, Stx2, and 16S rRNA genes
Target Size (bp) Primer Sequence 16S rRNA gene 206 VMP5 AAG AAG CAC CGG CTA ACT CC VMP6 CGC ATT TCA CCG CTA CAC C ST gene 169 SRM148a TCT GTA TTA TCT TTC CCC TCT TTT AGT C SRM148Ba GTA TTG TCT TTT TCA CCT TTC GCT C SRM149 GCA GGA TTA CAA CAC ART TCA CAG C LT gene 416 SRM152 CGA CAG ATT ATA CCG TGC TGA CTC SRM154 GTA ATC GTT CAT CAA TCA CAC CAA stx 1/stx2 313 5′ Stx1.1b CTG GAT TTA ATG TCG CAT AGT GG 5′ Stx2.1b CTG GCG TTA ATG GAG TTC AGT G SRM129 TGA TGA TGA CAA TTC AGT ATA ACT GCC AC - TABLE 2.
E. coli strains examined in this studya
Strain Serotype Group Virulence factor(s) Sourceb 35150 O157:H7 EHEC Stx1, Stx2 ATCC 35401 O78:H11 ETEC LT, ST ATCC 38 NAc Wild type NA FDA 04-02111 Ont:H52 STEC/ETEC ST, Stx1 MDP 04-01392 Ont:H52 STEC/ETEC ST, Stx1 MDP 103 NA ETEC ST, LT FDA 119 NA ETEC ST FDA 13C07 O26:H11 STEC Stx1 FDA TW01387 O111:H8 STEC Stx1 STEC Center TW05622 O138 STEC/ETEC ST, Stx2 STEC Center TW07509 Ond:H52d STEC/ETEC ST, Stx1 STEC Center TW10123 Ont:H52d STEC/ETEC ST, Stx1 STEC Center
