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Methods

High-Resolution Fluorometer for Mapping Microscale Phytoplankton Distributions

Mark J. Doubell, Laurent Seuront, Justin R. Seymour, Nicole L. Patten, James G. Mitchell
Mark J. Doubell
1School of Biological Sciences, Flinders University of South Australia, G.P.O. Box 2100, Adelaide, South Australia 5001, Australia
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  • For correspondence: Mark.Doubell@flinders.edu.au
Laurent Seuront
1School of Biological Sciences, Flinders University of South Australia, G.P.O. Box 2100, Adelaide, South Australia 5001, Australia
2Ecosystem Complexity Research Group, Station Marine de Wimereux, CNRS UMR 8013 ELICO, Univesité des Sciences et Technologies de Lille, 28 avenue Foch, 62930 Wimereux, France
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Justin R. Seymour
1School of Biological Sciences, Flinders University of South Australia, G.P.O. Box 2100, Adelaide, South Australia 5001, Australia
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Nicole L. Patten
3School of Environmental Science and Management, Southern Cross University, G.P.O. Box 157, Lismore, New South Wales 2480, Australia
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James G. Mitchell
1School of Biological Sciences, Flinders University of South Australia, G.P.O. Box 2100, Adelaide, South Australia 5001, Australia
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DOI: 10.1128/AEM.02959-05
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Figures

  • FIG. 1.
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    FIG. 1.

    Schematic diagram of the FluoroMAP fluorometer (A) and close-up of the fluorescence sensor and sample volume (B).

  • FIG. 2.
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    FIG. 2.

    FluoroMAP fluorescence intensities (•) (mean ± standard deviation; arbitrary units [a.u.]) and cell concentrations (□) (cells/μl) determined by flow cytometry during simulated blooms for cultures of P. lutheri (A1) and C. muelleri (B1). Representative cytograms show the fluorescence intensity (red fluorescence) and size (side scatter) of populations of P. lutheri (A2) and C. muelleri (B2).

  • FIG. 3.
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    FIG. 3.

    (A) High-resolution vertical profile of fluorescence intensity (arbitrary units [a.u.]) with depth in the Southern Ocean. (B) Enlarged section of the fluorescence microstructure shown in panel A between 23.7 and 24.7 m, showing a typical peak structure for a 1-m section.

  • FIG. 4.
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    FIG. 4.

    (A) High-resolution vertical profile of fluorescence intensity (arbitrary units [a.u.]) with depth in the eastern English Channel. (B) Enlarged section of the fluorescence microstructure shown in panel A between 6.5 and 7.5 m, showing a typical peak structure for a 1-m section.

  • FIG. 5.
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    FIG. 5.

    (A) Comparison of the FluoroMAP fluorescence intensity (arbitrary units [a.u.]) time series determined in situ at a depth of 17 m in Southern Ocean waters (gray) and in the laboratory for a solution of MilliQ water (black). (B) Close-up of the fluorescence intensity of the MilliQ signal in panel A between 20 and 30 s.

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High-Resolution Fluorometer for Mapping Microscale Phytoplankton Distributions
Mark J. Doubell, Laurent Seuront, Justin R. Seymour, Nicole L. Patten, James G. Mitchell
Applied and Environmental Microbiology Jun 2006, 72 (6) 4475-4478; DOI: 10.1128/AEM.02959-05

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High-Resolution Fluorometer for Mapping Microscale Phytoplankton Distributions
Mark J. Doubell, Laurent Seuront, Justin R. Seymour, Nicole L. Patten, James G. Mitchell
Applied and Environmental Microbiology Jun 2006, 72 (6) 4475-4478; DOI: 10.1128/AEM.02959-05
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  • Top
  • Article
    • ABSTRACT
    • FluoroMAP.
    • Chlorophyll concentration in cultures.
    • Cell-to-cell fluorescence variability.
    • Application in contrasting marine systems.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
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KEYWORDS

phytoplankton

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