Article Figures & Data
Figures
- FIG. 1.
tceA gene expression profile of a pure culture of Dehalococcoides ethenogenes 195 in the presence and absence of TCE over 24 h. Measurements of tceA transcripts per tceA gene at each time point are normalized to the value at time zero to obtain the relative changes. Data are calculated from averages of triplicate qPCRs and RT-qPCRs, and error bars are smaller than the box symbol.
- FIG. 2.
Reductive dechlorination of chlorinated ethenes (solid lines) and expression profile of the tceA and vcrA genes (dashed lines) over a feeding cycle for ANAS. Expression measurements are calculated from averages of triplicate qPCRs and RT-qPCRs, and error bars represent 1 standard deviation.
- FIG. 3.
Decay profile of tceA transcripts when chlorinated ethenes were stripped from the culture after 24 h of active dechlorination.
- FIG. 4.
(A) tceA gene expression profile with and without the influence of chloramphenicol. (B) Initial dechlorination rate of TCE in the presence of chloramphenicol after the establishment of different transcript levels in five respective cultures. Rate measurements are normalized to the tceA gene copy number. The error bars for the rate measurement represent the 95% confidence interval of the slope of TCE dechlorination over 10 h. (C) Dechlorination activity of the ANAS enrichment culture over three consecutive days as represented by the rate of production of the TCE daughter products cDCE and VC every 24 h. Error bars represent the 95% confidence interval. The inset graph shows tceA gene expression during the 3 days of active dechlorination.
Tables
- TABLE 1.
Dechlorination activity
No. of days without chlorinated ethenes Mass amt (μmol) ofa: TCE dechlorinated VC produced 0 3.0 0.7 4 2.1 0.3 9 1.7 0.2 15 1.5 0 42 1.0 0 ↵ a Data represent mass amounts of TCE dechlorinated and VC produced in ANAS enrichment culture that had been starved of chlorinated ethenes for various durations. Data were measured after 14 h. cDCE was produced during each day of sampling but was not included in this table.
