Article Figures & Data
Figures
- FIG. 1.
Diagram of systems used for electrolytic treatment of biofilms. The coupon covered with biofilm (dark gray) and a clean coupon or wire (light gray) were immersed in a beaker (A, side view) or open dish (B, top view) containing 10 g liter−1 NaCl. The two metal pieces were connected to a 6-V battery with small jumper cables.
- FIG. 2.
Reduction in biofilm viable cell numbers during electrolytic treatment of biofilms connected as the anode (•) or cathode (○). The error indicated is the standard deviation.
- FIG. 3.
Patterns of respiratory activity in S. epidermidis biofilms visualized by staining with the respiratory indicator CTC (red) and the DNA dye DAPI (green). These are frozen cross sections that are representative of the patterns seen in duplicate experiments. In each panel, the substratum was formerly closer to the lower left corner and the biofilm-bulk fluid interface was closer to the upper right corner. Red color indicates that highly actively respiring cells are present. Yellow color, obtained by the overlap of red and green, indicates the presence of cells with an intermediate level of respiratory activity. Green color, indicating the presence of DNA but absence of CTC staining, reveals regions of very low respiratory activity. (a) Untreated control; (b) biofilm treated as the anode; (c) biofilm treated as the cathode.
