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Methods

High-Resolution DNA Melt Curve Analysis of the Clustered, Regularly Interspaced Short-Palindromic-Repeat Locus of Campylobacter jejuni

Erin P. Price, Helen Smith, Flavia Huygens, Philip M. Giffard
Erin P. Price
1Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia
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Helen Smith
2Queensland Health Scientific Services, Coopers Plains, Australia
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Flavia Huygens
1Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia
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Philip M. Giffard
1Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia
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  • For correspondence: p.giffard@qut.edu.au
DOI: 10.1128/AEM.02702-06
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Article Information

DOI 
https://doi.org/10.1128/AEM.02702-06
PubMed 
17400785

Published By 
American Society for Microbiology Journals
History 
  • Received November 20, 2006
  • Accepted March 17, 2007
  • Published online May 10, 2007.

Copyright & Usage 
Copyright © 2007 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (March 30, 2007).
  • You are viewing the most recent version of this article.

Author Information

  1. Erin P. Price1,
  2. Helen Smith2,
  3. Flavia Huygens1 and
  4. Philip M. Giffard1*
  1. 1Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia
  2. 2Queensland Health Scientific Services, Coopers Plains, Australia
  • ↵* Corresponding author. Mailing address: Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Cnr Blamey St. and Musk Ave., Kelvin Grove, Queensland 4059, Australia. Phone: 61 7 3138 6194. Fax: 61 7 3138 6030. E-mail: p.giffard{at}qut.edu.au
  • † Supplemental material for this article may be found at http://aem.asm.org/ .

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High-Resolution DNA Melt Curve Analysis of the Clustered, Regularly Interspaced Short-Palindromic-Repeat Locus of Campylobacter jejuni
Erin P. Price, Helen Smith, Flavia Huygens, Philip M. Giffard
Applied and Environmental Microbiology May 2007, 73 (10) 3431-3436; DOI: 10.1128/AEM.02702-06

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High-Resolution DNA Melt Curve Analysis of the Clustered, Regularly Interspaced Short-Palindromic-Repeat Locus of Campylobacter jejuni
Erin P. Price, Helen Smith, Flavia Huygens, Philip M. Giffard
Applied and Environmental Microbiology May 2007, 73 (10) 3431-3436; DOI: 10.1128/AEM.02702-06
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  • Top
  • Article
    • ABSTRACT
    • CRISPR detection in C. jejuni.
    • CRISPR sequencing.
    • HRM analysis of the CRISPR locus.
    • Comparison of genotyping methods with CRISPR HRM.
    • Nucleotide sequence accession numbers.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
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  • PDF

KEYWORDS

Bacterial Typing Techniques
Campylobacter jejuni
DNA, Bacterial
Nucleic Acid Denaturation
Repetitive Sequences, Nucleic Acid

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