Article Figures & Data
Figures
- FIG. 1.
Transcript levels for genes in the genome of D. ethenogenes strain 195 annotated as potential reductive dehalogenases, including tceA (DET0079) and pceA (DET0318), in cells grown on PCE, TCE, or 2,3-DCP. All transcript levels were normalized to that of rpoB (R′), and error bars represent standard deviations.
- FIG. 2.
(A) Regions upstream of transcription starts determined by 5′-RACE for tceA, pceA, infA, and DET1407 from D. ethenogenes strain 195 and the region upstream of vcrA from strain VS (VSvcrA) (26). Underlined bases represent potential −10 regions, TG motifs upstream of −10, or potential −35 regions. (B) Transcription leader regions upstream of vcrA and tceA showing regions of high sequence identity.
- FIG. 3.
D. ethenogenes protein coverage obtained from PCE-grown cells (PCE; white bars) or 2,3-DCP-grown cells (CPs; gray bars). Peptide fragments identified by electron spray ionization and matrix-assisted laser desorption ionization MS/MS of the five most highly expressed proteins identified in cell membrane-enriched fractions.
- FIG. 4.
Reductive dehalogenation activities of resting cells of D. ethenogenes strain 195 grown on PCE or TCE. (A) PCE dechlorination by PCE-grown cells. (B) 2,3-DCP dechlorination by PCE-grown cells. (C) PCE dechlorination by 2,3-DCP-grown cells. (D) 2,3-DCP dechlorination by 2,3-DCP-grown cells. Each graph shows results for a representative vial from experiments run in triplicate. 3-MCP, 3-monochlorophenol.
Additional Files
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
List of PCR primers used in this study (Table S1).
Zipped PDF document, 33K.
- Supplemental file 1
-
List of PCR primers used in this study (Table S1).
