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Microbial Ecology

Rhizobacterial Volatiles Affect the Growth of Fungi and Arabidopsis thaliana

Anja Vespermann, Marco Kai, Birgit Piechulla
Anja Vespermann
University of Rostock, Department of Biological Sciences, Albert-Einstein-Str. 3, 18059 Rostock, Germany
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Marco Kai
University of Rostock, Department of Biological Sciences, Albert-Einstein-Str. 3, 18059 Rostock, Germany
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Birgit Piechulla
University of Rostock, Department of Biological Sciences, Albert-Einstein-Str. 3, 18059 Rostock, Germany
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  • For correspondence: birgit.piechulla@uni-rostock.de
DOI: 10.1128/AEM.01078-07
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    FIG. 1.

    Cocultivation of rhizobacteria with fungi or A. thaliana in the presence or absence of charcoal. Nutrient broth (left panels) or overnight rhizobacterial cultures (20 or 50 μl) (middle and right panels) were plated in one compartment of a tripartite petri dish. After 2 days of incubation, a fungal mycelium plug (P. carneus [A] or R. solani [B]) or 10 A. thaliana seedlings with or without charcoal (right and middle panels, respectively) were placed in the other compartments, and incubation was continued for 4 days for the fungi or for 14 days for A. thaliana.

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    FIG. 2.

    Determination of growth inhibition. Mycelial growth inhibition or promotion was calculated after 4 days of cocultivation. The fresh weight of A. thaliana was determined after 14 days of cocultivation. Experiments were repeated three times, and each experiment comprised three to seven replicates with each bacterial isolate. The error bars indicate standard errors. The bacterial isolates used were B. subtilis B2g (B.s.), B. cepacia 1S18 (B.c.), P. fluorescens L13-6-12 (P.f.), P. trivialis 3Re2-7 (P.t.), S. odorifera 4Rx13 (S.o.), S. plymuthica 3Re4-18 (S.p.), S. plymuthica HRO C48 (S.p.H.), S. epidermidis 2P13-18 (S.e.), S. maltophilia R3089 (S.m.), and S. rhizophila P69 (S.r.). The soilborne fungi used were F. culmorum PR 19-12-11, F. solani, M. bolleyi PR 5-11-6, P. carneus PR 16-10-1, Penicillium sp. strain 2-1-20, Penicillium waksmanii PR 17-11-8, P. betae, Phoma eupyrena PC 17-12-10, R. solani AG3, S. sclerotiorum, Trichoderma strictipile PC26-12-6, and V. dahliae V25. In addition, A. niger and N. crassa wild-type 1202A were used.

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Rhizobacterial Volatiles Affect the Growth of Fungi and Arabidopsis thaliana
Anja Vespermann, Marco Kai, Birgit Piechulla
Applied and Environmental Microbiology Aug 2007, 73 (17) 5639-5641; DOI: 10.1128/AEM.01078-07

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Rhizobacterial Volatiles Affect the Growth of Fungi and Arabidopsis thaliana
Anja Vespermann, Marco Kai, Birgit Piechulla
Applied and Environmental Microbiology Aug 2007, 73 (17) 5639-5641; DOI: 10.1128/AEM.01078-07
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KEYWORDS

antifungal agents
Arabidopsis
Bacillus
fungi
Gram-negative bacteria
Plant Growth Regulators
Plant Roots

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