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Enzymology and Protein Engineering

Lactococcus lactis Gene yjgB Encodes a γ-d-Glutaminyl-l-Lysyl- Endopeptidase Which Hydrolyzes Peptidoglycan

Yulia Redko, Pascal Courtin, Christine Mézange, Carine Huard, Marie-Pierre Chapot-Chartier
Yulia Redko
INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
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Pascal Courtin
INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
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Christine Mézange
INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
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Carine Huard
INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
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Marie-Pierre Chapot-Chartier
INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
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  • For correspondence: Marie-Pierre.Chapot@jouy.inra.fr
DOI: 10.1128/AEM.00705-07
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ABSTRACT

YjgB is one of five peptidoglycan hydrolases previously identified in Lactococcus lactis. Analysis of its amino acid sequence revealed that YjgB contains an NlpC/P60 domain, whereas no specific cell wall binding domain or motif could be identified. The NlpC/P60 family is characterized by three conserved residues, a cysteine, a histidine, and a polar residue. In agreement with the presence of a Cys residue in the catalytic site of YjgB, its enzymatic activity was enhanced in the presence of dithiothreitol. Peptidoglycan-hydrolyzing activity of YjgB was detected in growing cells of an L. lactis strain overexpressing YjgB, as revealed by the presence of disaccharide (DS)-dipeptide in the muropeptide composition of the overexpressing strain. YjgB hydrolyzes the peptide chains of L. lactis muropeptides between γ-d-Gln and l-Lys residues. Its hydrolytic activity was detected on DSs with tetra- and pentapeptide chains, whereas hydrolytic activity was very low on DS-tripeptides. Thus, we demonstrated that YjgB is an endopeptidase which cleaves γ-d-Gln-l-Lys bonds in peptide chains of L. lactis peptidoglycan.

  • Copyright © 2007 American Society for Microbiology
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Lactococcus lactis Gene yjgB Encodes a γ-d-Glutaminyl-l-Lysyl- Endopeptidase Which Hydrolyzes Peptidoglycan
Yulia Redko, Pascal Courtin, Christine Mézange, Carine Huard, Marie-Pierre Chapot-Chartier
Applied and Environmental Microbiology Sep 2007, 73 (18) 5825-5831; DOI: 10.1128/AEM.00705-07

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Lactococcus lactis Gene yjgB Encodes a γ-d-Glutaminyl-l-Lysyl- Endopeptidase Which Hydrolyzes Peptidoglycan
Yulia Redko, Pascal Courtin, Christine Mézange, Carine Huard, Marie-Pierre Chapot-Chartier
Applied and Environmental Microbiology Sep 2007, 73 (18) 5825-5831; DOI: 10.1128/AEM.00705-07
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KEYWORDS

Endopeptidases
Genes, Bacterial
Lactococcus lactis
peptidoglycan

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