Article Figures & Data
Figures
- FIG. 1.
Phylogenetic tree representing affiliation of 16S rRNA clone sequences of Bacteria retrieved from granule samples (OTU numbers). The tree was generated by using nearly full-length 16S rRNA gene sequences and the neighbor-joining method. The scale bar represents 5% estimated divergence. The numbers at the nodes are bootstrap values (1,000 replicates) with more than 50% bootstrap support.
- FIG. 2.
Phylogenetic tree representing affiliation of 16S rRNA clone sequences of Archaea retrieved from granule samples (OTU numbers). The tree was generated by using nearly full-length 16S rRNA gene sequences and the neighbor-joining method. The scale bar represents 5% estimated divergence. The numbers at the nodes are bootstrap values (1,000 replicates) with more than 50% bootstrap support.
- FIG. 3.
Confocal laser scanning microscope images of thin sections of the anaerobic granules showing the in situ spatial organization of bacteria and archaea. (A) DAPI-stained and differential interference contrast images. (B) FISH with TRITC-labeled probe ARC915 and the FITC-labeled EUB338-mixed probe. (C) FISH with FITC-labeled probe GNSB-941. (D) FISH with TRITC-labeled probe ARC915 and FITC-labeled probe BET42a. (E) FISH with TRITC-labeled probe ARC915 and FITC-labeled probe LGC354. (F) FISH with TRITC-labeled probe ARC915 and FITC-labeled probe ALF968. (G) FISH with TRITC-labeled probe MX825 and FITC-labeled probe HGC69A. Scale bars indicate 200 μm (panels A and B) and 50 μm (panels C through G). For panels B through G, TRITC-labeled probes are red and FITC-labeled probes are green.
- FIG. 4.
Concentration profiles of pH, ORP, CH4, and H2 in the anaerobic granule. Each profile value is the average of three measurements, and the error bars represent the standard deviations of triplicate measurements. Zero on the vertical axis corresponds to the surface of the granule.
- FIG. 5.
Spatial distributions and magnitudes of the net volumetric production rates of CH4 and H2. The rates were calculated based on the corresponding concentration profiles shown in Fig. 4. Each profile value is the average of three measurements, and the error bars represent the standard deviations of triplicate measurements. Negative values indicate consumption rates. Zero on the vertical axis corresponds to the surface of the granule.
- FIG. 6.
A typical transient H2 concentration profile measured at the center of the granule inhibited by chloroform. Points (○) indicate H2 concentrations measured, and the solid line is a theoretical curve.
Additional Files
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
Typical calibration curve for a microscale biosensor for CH4 (Fig. S1)
Zipped PowerPoint document, 20K.
- Supplemental file 1
-
Typical calibration curve for a microscale biosensor for CH4 (Fig. S1)
