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Mycology

Establishment of Agrobacterium tumefaciens-Mediated Transformation of an Oleaginous Fungus, Mortierella alpina 1S-4, and Its Application for Eicosapentaenoic Acid Producer Breeding

Akinori Ando, Yosuke Sumida, Hiroaki Negoro, Dian Anggraini Suroto, Jun Ogawa, Eiji Sakuradani, Sakayu Shimizu
Akinori Ando
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
2Research Division of Microbial Sciences, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
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Yosuke Sumida
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
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Hiroaki Negoro
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
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Dian Anggraini Suroto
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
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Jun Ogawa
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
2Research Division of Microbial Sciences, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
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Eiji Sakuradani
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
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Sakayu Shimizu
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
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  • For correspondence: sim@kais.kyoto-u.ac.jp
DOI: 10.1128/AEM.00648-09
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Article Information

DOI 
https://doi.org/10.1128/AEM.00648-09
PubMed 
19581481

Published By 
American Society for Microbiology Journals
History 
  • Received March 19, 2009
  • Accepted June 25, 2009
  • Published online August 24, 2009.

Copyright & Usage 
Copyright © 2009 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (July 06, 2009).
  • You are viewing the most recent version of this article.

Author Information

  1. Akinori Ando1,2,
  2. Yosuke Sumida1,
  3. Hiroaki Negoro1,
  4. Dian Anggraini Suroto1,
  5. Jun Ogawa1,2,
  6. Eiji Sakuradani1 and
  7. Sakayu Shimizu1*
  1. 1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
  2. 2Research Division of Microbial Sciences, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan
  • ↵* Corresponding author. Mailing address: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan. Phone: 81 75 753 6122. Fax: 81 75 753 6128. E-mail: sim{at}kais.kyoto-u.ac.jp

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Establishment of Agrobacterium tumefaciens-Mediated Transformation of an Oleaginous Fungus, Mortierella alpina 1S-4, and Its Application for Eicosapentaenoic Acid Producer Breeding
Akinori Ando, Yosuke Sumida, Hiroaki Negoro, Dian Anggraini Suroto, Jun Ogawa, Eiji Sakuradani, Sakayu Shimizu
Applied and Environmental Microbiology Aug 2009, 75 (17) 5529-5535; DOI: 10.1128/AEM.00648-09

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Establishment of Agrobacterium tumefaciens-Mediated Transformation of an Oleaginous Fungus, Mortierella alpina 1S-4, and Its Application for Eicosapentaenoic Acid Producer Breeding
Akinori Ando, Yosuke Sumida, Hiroaki Negoro, Dian Anggraini Suroto, Jun Ogawa, Eiji Sakuradani, Sakayu Shimizu
Applied and Environmental Microbiology Aug 2009, 75 (17) 5529-5535; DOI: 10.1128/AEM.00648-09
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KEYWORDS

Agrobacterium tumefaciens
Eicosapentaenoic Acid
Gene Transfer Techniques
genetic engineering
Mortierella
Transformation, Genetic

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