Food Microbiology

Comparative Analysis of Attachment of Shiga-Toxigenic Escherichia coli and Salmonella Strains to Cultured HT-29 and Caco-2 Cell Lines

Glen E. Mellor, Rebecca M. Goulter, T. W. Raymond Chia, Gary A. Dykes
DOI: 10.1128/AEM.02232-08

Article Figures & Data

Tables

  • TABLE 1.

    S. enterica and E. coli isolate numbers, serovars or serotypes, and sources

    Isolate no.Serovar or serotypeSource
    S. enterica
        ATCCa 14028TyphimuriumControl
        S1380aTyphimuriumChicken rinse
        S1567aTyphimuriumChicken rinse
        S1672TyphimuriumChicken
        S1673TyphimuriumChicken
        S1674TyphimuriumChicken
        S1297aVirchowChicken rinse
        S1680VirchowChicken
        S1457bInfantisChicken rinse
        S1677InfantisChicken
        S1679InfantisChicken
        S1296aSofiaChicken rinse
        S1309aSofiaChicken rinse
        S1540bSofiaChicken rinse
        S1628SofiaChicken
        S1629SofiaChicken litter
        S1630SofiaChicken
        S1634SofiaChicken meat
        S1635SofiaHuman
        S1636fSofiaLab generated
        S1637SofiaHuman
        S1638SofiaChicken
        S1640SofiaChicken
        S1663SofiaChicken
        S1667SofiaChicken
    E. coli
        EC1812bO157:H7Human bloody diarrheac
        EC1820bO157:H7Human deathc
        EC1596bO157:H7EDL933c,d
        EC119bO157:H7Beef carcass
        EC200bO157:H7Beef carcass
        EC514bO157:H7Boneless beef
        EC623bO157:H7Beef cattle hide
        EC1814bO157:H−Human bloody diarrheac
        EC183bO157:H−Sheep feces
        EC329bO157:H−Beef cattle feces
        EC516bO157:H−Calf feces
        EC26bO111:H−Calf feces
        EC473bO26:H111Dairy cattle feces
        EC2141bO91:H21Cattle feces
        EC2170bO165:H25Cattle feces
        EC2177bO113:H21Cattle feces
        EC2214bO174:H21Cattle feces
        EC1598O13rel:H4eUnknown
        EC1858O1:H7ATCCa 11775
        EC614O157:HRgBeef cattle feces

    • ↵ a ATCC, American Type Culture Collection.

    • ↵ b Contains at least one of the stx1, stx2, eae, or ehxA genes as previously reported by Rivas et al. (19).

    • ↵ c Strains received from R. Robins-Browne, University of Melbourne, Australia.

    • ↵ d Strain implicated in outbreak.

    • ↵ e Isolate is related (rel) to the O13 serotype and produced a weak titer with the O13 serotype only.

    • ↵ f Mutant strain of S1635.

    • ↵ g H antigen is rough.

  • TABLE 2.

    S. enterica and E. coli attachment per 100 Caco-2 or HT-29 intestinal cells

    Isolate no.Mean (SD) attachment (CFU/100 cells) of isolate to:
    Caco-2HT-29
    S. enterica
        ATCCa 140281,174 (307)182 (89)
        S1380a764 (50)43 (5)
        S1567a547 (158)32 (2)
        S1672628 (231)33 (6)
        S1673588 (93)20 (1)
        S1674448 (136)17 (7)
        S1297a826 (145)36 (19)
        S16801,002 (41)37 (16)
        S1457b661 (49)33 (4)
        S16771,381 (159)25 (7)
        S1679762 (221)25 (5)
        S1296a660 (77)47 (12)
        S1309a1,079 (506)83 (77)
        S1540b915 (223)42 (20)
        S1628668 (147)45 (37)
        S16291,487 (814)49 (21)
        S1630805 (171)36 (8)
        S1634591 (114)36 (8)
        S1635991 (325)38 (28)
        S1636f992 (243)33 (12)
        S1637828 (64)35 (7)
        S16381,045 (113)36 (7)
        S16401,301 (771)36 (17)
        S1663632 (317)62 (38)
        S1667647 (124)40 (19)
    E. coli
        EC1812b,c1,739 (762)218 (69)
        EC1820b,c1,229 (578)266 (101)
        EC1596b,c,d1,472 (244)257 (71)
        EC119b1,131 (386)327 (212)
        EC200b888 (350)151 (84)
        EC514b874 (115)140 (60)
        EC623b1,988 (437)253 (93)
        EC1814b1,821 (565)240 (123)
        EC183b1,911 (1,147)277 (131)
        EC329b864 (146)272 (149)
        EC516b1,595 (357)401 (252)
        EC26b1,276 (277)242 (172)
        EC473b923 (521)289 (116)
        EC2141b2,015 (755)315 (115)
        EC2170b1,143 (152)296 (230)
        EC2177b1,185 (272)147 (136)
        EC2214b863 (219)258 (137)
        EC1598e817 (32)142 (82)
        EC1858a1,927 (691)181 (104)
        EC614g3,336 (479)620 (158)

    • ↵ a ATCC, American Type Culture Collection.

    • ↵ b Contains at least one of the stx1, stx2, eae, or ehxA genes as previously reported by Rivas et al. (19).

    • ↵ c Strains received from R. Robins-Browne, University of Melbourne, Australia.

    • ↵ d Strain implicated in outbreak.

    • ↵ e Isolate is related (rel) to the O13 serotype and produced a weak titer with the O13 serotype only.

    • ↵ f Mutant strain of S1635.

    • ↵ g H antigen is rough.

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KEYWORDS

bacterial adhesion
Epithelial Cells
Salmonella
Shiga-toxigenic Escherichia coli

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