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Methods

Broad-Host-Range Plasmids for Red Fluorescent Protein Labeling of Gram-Negative Bacteria for Use in the Zebrafish Model System

John T. Singer, Ryan T. Phennicie, Matthew J. Sullivan, Laura A. Porter, Valerie J. Shaffer, Carol H. Kim
John T. Singer
1Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469
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  • For correspondence: jsinger@maine.edu
Ryan T. Phennicie
1Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469
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Matthew J. Sullivan
1Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469
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Laura A. Porter
1Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469
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Valerie J. Shaffer
1Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469
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Carol H. Kim
1Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469
2Graduate School for Biomedical Sciences, ESRB/Barrows Hall, University of Maine, Orono, Maine 04469
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DOI: 10.1128/AEM.01679-09
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Article Information

DOI 
https://doi.org/10.1128/AEM.01679-09
PubMed 
20363780

Published By 
American Society for Microbiology Journals
History 
  • Received July 15, 2009
  • Accepted March 26, 2010
  • Published online May 19, 2010.

Copyright & Usage 
Copyright © 2010 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (April 02, 2010).
  • You are viewing the most recent version of this article.

Author Information

  1. John T. Singer1*,
  2. Ryan T. Phennicie1,
  3. Matthew J. Sullivan1‡,
  4. Laura A. Porter1§,
  5. Valerie J. Shaffer1¶ and
  6. Carol H. Kim1,2
  1. 1Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, Maine 04469
  2. 2Graduate School for Biomedical Sciences, ESRB/Barrows Hall, University of Maine, Orono, Maine 04469
  • ↵* Corresponding author. Mailing address: Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, ME 04469. Phone: (207) 581-2808. Fax: (207) 581-2801. E-mail: jsinger{at}maine.edu
  • ↵‡ Present address: Quincy College, Quincy, MA 02171.; § Present address: : Department of Microbiology, University of Virginia, Charlottesville, VA 22908.; ¶ Present address: : The Jackson Laboratory, Bar Harbor, ME 04609.

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Broad-Host-Range Plasmids for Red Fluorescent Protein Labeling of Gram-Negative Bacteria for Use in the Zebrafish Model System
John T. Singer, Ryan T. Phennicie, Matthew J. Sullivan, Laura A. Porter, Valerie J. Shaffer, Carol H. Kim
Applied and Environmental Microbiology May 2010, 76 (11) 3467-3474; DOI: 10.1128/AEM.01679-09

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Broad-Host-Range Plasmids for Red Fluorescent Protein Labeling of Gram-Negative Bacteria for Use in the Zebrafish Model System
John T. Singer, Ryan T. Phennicie, Matthew J. Sullivan, Laura A. Porter, Valerie J. Shaffer, Carol H. Kim
Applied and Environmental Microbiology May 2010, 76 (11) 3467-3474; DOI: 10.1128/AEM.01679-09
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KEYWORDS

Gram-negative bacteria
host-pathogen interactions
Luminescent Proteins
molecular biology
plasmids
Staining and Labeling

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