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Methods

Comparison of Normalization Methods for Construction of Large, Multiplex Amplicon Pools for Next-Generation Sequencing

J. Kirk Harris, Jason W. Sahl, Todd A. Castoe, Brandie D. Wagner, David D. Pollock, John R. Spear
J. Kirk Harris
1Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado 80045
5Consortium for Comparative Genomics, University of Colorado Denver, Aurora, Colorado 80045
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  • For correspondence: jonathan.harris@ucdenver.edu
Jason W. Sahl
2Department of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401
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Todd A. Castoe
3Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045
5Consortium for Comparative Genomics, University of Colorado Denver, Aurora, Colorado 80045
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Brandie D. Wagner
4Department of Biostatistics and Informatics, Colorado School of Public Health, Aurora, University of Colorado Denver, Aurora, Colorado 80045
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David D. Pollock
3Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045
5Consortium for Comparative Genomics, University of Colorado Denver, Aurora, Colorado 80045
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John R. Spear
2Department of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401
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DOI: 10.1128/AEM.02585-09
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Article Information

DOI 
https://doi.org/10.1128/AEM.02585-09
PubMed 
20418443

Published By 
American Society for Microbiology Journals
History 
  • Received October 23, 2009
  • Accepted April 18, 2010
  • Published online June 4, 2010.

Copyright & Usage 
Copyright © 2010 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (April 23, 2010).
  • You are viewing the most recent version of this article.

Author Information

  1. J. Kirk Harris1,5*,
  2. Jason W. Sahl2,
  3. Todd A. Castoe3,5,
  4. Brandie D. Wagner4,
  5. David D. Pollock3,5 and
  6. John R. Spear2
  1. 1Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado 80045
  2. 2Department of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401
  3. 3Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045
  4. 4Department of Biostatistics and Informatics, Colorado School of Public Health, Aurora, University of Colorado Denver, Aurora, Colorado 80045
  5. 5Consortium for Comparative Genomics, University of Colorado Denver, Aurora, Colorado 80045
  • ↵* Corresponding author. Mailing address: Department of Pediatrics, University of Colorado Denver, 13123 E. 16th Ave., B395, Aurora, CO 80045. Phone: (720) 777-4943. Fax: (720) 777-7284. E-mail: jonathan.harris{at}ucdenver.edu
  • † Supplemental material for this article may be found at http://aem.asm.org/ .

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Comparison of Normalization Methods for Construction of Large, Multiplex Amplicon Pools for Next-Generation Sequencing
J. Kirk Harris, Jason W. Sahl, Todd A. Castoe, Brandie D. Wagner, David D. Pollock, John R. Spear
Applied and Environmental Microbiology Jun 2010, 76 (12) 3863-3868; DOI: 10.1128/AEM.02585-09

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Comparison of Normalization Methods for Construction of Large, Multiplex Amplicon Pools for Next-Generation Sequencing
J. Kirk Harris, Jason W. Sahl, Todd A. Castoe, Brandie D. Wagner, David D. Pollock, John R. Spear
Applied and Environmental Microbiology Jun 2010, 76 (12) 3863-3868; DOI: 10.1128/AEM.02585-09
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KEYWORDS

Gene Library
metagenomics
Sequence Analysis, DNA
Staining and Labeling

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