Characterization of a Thermostable Short-Chain Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Thermococcus sibiricus

Tatiana N. Stekhanova; Andrey V. Mardanov; Ekaterina Y. Bezsudnova; Vadim M. Gumerov; Nikolai V. Ravin; Konstantin G. Skryabin; Vladimir O. Popov

doi:10.1128/AEM.02797-09

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Characterization of a Thermostable Short-Chain Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Thermococcus sibiricus

Tatiana N. Stekhanova, Andrey V. Mardanov, Ekaterina Y. Bezsudnova, Vadim M. Gumerov, Nikolai V. Ravin, Konstantin G. Skryabin, Vladimir O. Popov

Tatiana N. Stekhanova

1Bach Institute of Biochemistry Russian Academy of Sciences, Leninsky pr. 33, Moscow 119071, Russia

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For correspondence: tstekh@yandex.ru

Andrey V. Mardanov

2Centre Bioengineering, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, Bld. 7-1, Moscow 117312, Russia

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Ekaterina Y. Bezsudnova

1Bach Institute of Biochemistry Russian Academy of Sciences, Leninsky pr. 33, Moscow 119071, Russia

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Vadim M. Gumerov

2Centre Bioengineering, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, Bld. 7-1, Moscow 117312, Russia

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Nikolai V. Ravin

2Centre Bioengineering, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, Bld. 7-1, Moscow 117312, Russia

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Konstantin G. Skryabin

2Centre Bioengineering, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, Bld. 7-1, Moscow 117312, Russia

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Vladimir O. Popov

1Bach Institute of Biochemistry Russian Academy of Sciences, Leninsky pr. 33, Moscow 119071, Russia

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DOI: 10.1128/AEM.02797-09

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ABSTRACT

Short-chain alcohol dehydrogenase, encoded by the gene Tsib_0319 from the hyperthermophilic archaeon Thermococcus sibiricus, was expressed in Escherichia coli, purified and characterized as an NADPH-dependent enantioselective oxidoreductase with broad substrate specificity. The enzyme exhibits extremely high thermophilicity, thermostability, and tolerance to organic solvents and salts.

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Characterization of a Thermostable Short-Chain Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Thermococcus sibiricus

Tatiana N. Stekhanova, Andrey V. Mardanov, Ekaterina Y. Bezsudnova, Vadim M. Gumerov, Nikolai V. Ravin, Konstantin G. Skryabin, Vladimir O. Popov

Applied and Environmental Microbiology Jun 2010, 76 (12) 4096-4098; DOI: 10.1128/AEM.02797-09

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Characterization of a Thermostable Short-Chain Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Thermococcus sibiricus

Tatiana N. Stekhanova, Andrey V. Mardanov, Ekaterina Y. Bezsudnova, Vadim M. Gumerov, Nikolai V. Ravin, Konstantin G. Skryabin, Vladimir O. Popov

Applied and Environmental Microbiology Jun 2010, 76 (12) 4096-4098; DOI: 10.1128/AEM.02797-09

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KEYWORDS

alcohol dehydrogenase

Thermococcus

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Characterization of a Thermostable Short-Chain Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Thermococcus sibiricus

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