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Methods

Use of Amino Acids as Inducers for High-Level Protein Expression in the Single-Protein Production System

S. Thangminlal Vaiphei, Lili Mao, Tsutomu Shimazu, Jung-Ho Park, Masayori Inouye
S. Thangminlal Vaiphei
Department of Biochemistry, Robert Wood Johnson Medical School, and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, New Jersey 08854
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Lili Mao
Department of Biochemistry, Robert Wood Johnson Medical School, and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, New Jersey 08854
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Tsutomu Shimazu
Department of Biochemistry, Robert Wood Johnson Medical School, and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, New Jersey 08854
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Jung-Ho Park
Department of Biochemistry, Robert Wood Johnson Medical School, and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, New Jersey 08854
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Masayori Inouye
Department of Biochemistry, Robert Wood Johnson Medical School, and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, New Jersey 08854
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  • For correspondence: Inouye@umdnj.edu
DOI: 10.1128/AEM.00815-10
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Article Information

DOI 
https://doi.org/10.1128/AEM.00815-10
PubMed 
20656860

Published By 
American Society for Microbiology Journals
History 
  • Received April 1, 2010
  • Accepted July 12, 2010
  • Published online September 7, 2010.

Copyright & Usage 
Copyright © 2010 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (July 23, 2010).
  • You are viewing the most recent version of this article.

Author Information

  1. S. Thangminlal Vaiphei†,
  2. Lili Mao†,
  3. Tsutomu Shimazu,
  4. Jung-Ho Park and
  5. Masayori Inouye*
  1. Department of Biochemistry, Robert Wood Johnson Medical School, and Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, New Jersey 08854
  • ↵† These authors equally contributed to this work.

  • ↵* Corresponding author. Mailing address: Department of Biochemistry, Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854. Phone: (732) 235-4115. Fax: (732) 235-4559. E-mail: Inouye{at}umdnj.edu
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Use of Amino Acids as Inducers for High-Level Protein Expression in the Single-Protein Production System
S. Thangminlal Vaiphei, Lili Mao, Tsutomu Shimazu, Jung-Ho Park, Masayori Inouye
Applied and Environmental Microbiology Sep 2010, 76 (18) 6063-6068; DOI: 10.1128/AEM.00815-10

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Use of Amino Acids as Inducers for High-Level Protein Expression in the Single-Protein Production System
S. Thangminlal Vaiphei, Lili Mao, Tsutomu Shimazu, Jung-Ho Park, Masayori Inouye
Applied and Environmental Microbiology Sep 2010, 76 (18) 6063-6068; DOI: 10.1128/AEM.00815-10
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KEYWORDS

bioreactors
DNA-Binding Proteins
Endoribonucleases
Escherichia coli
Escherichia coli Proteins
Isotope Labeling
Protein Biosynthesis
protein engineering

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