FIG. 5.
FIG. 5.
R. palustris H2 production by thiosulfate oxidation under growing and nongrowing conditions. Nongrowing R. palustris (green pathways) produce H2 using nitrogenase without the need to produce biosynthetic precursors necessary for growth (yellow pathways). Cells take up bicarbonate from the external environment and convert it to carbon dioxide, which is the substrate for Calvin cycle reactions. The theoretical stoichiometry for maximum H2 production from nongrowing cells using thiosulfate is given in the equation.