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Applied and Environmental Microbiology
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Food Microbiology

Binding of Virus-Like Particles of Norwalk Virus to Romaine Lettuce Veins

Kamal M. Gandhi, Robert E. Mandrell, Peng Tian
Kamal M. Gandhi
Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California 94710
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Robert E. Mandrell
Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California 94710
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Peng Tian
Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California 94710
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  • For correspondence: peng.tian@ars.usda.gov
DOI: 10.1128/AEM.01566-10
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  • FIG. 1.
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    FIG. 1.

    Strips of romaine lettuce were viewed under a dissecting microscope (A and B) or a confocal microscope (C and D), after the application of rNVLP (A and C) or PBS (B and D), followed by antibody staining. Red, autofluorescence of romaine leaves; green, rNVLP.

  • FIG. 2.
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    FIG. 2.

    Wells of a plate were coated with PBS, PGM, or the listed dilution amount of RE, and rNVLP binding was assayed by ELISA. Error bars represent standard deviations (SD).

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    FIG. 3.

    Comparison between rNVLP binding to PGM and RE was assayed by ELISA. (A) The involvement of sugar and protein moieties in rNVLP binding was determined by treatment of PGM or RE with KIO4 or after being boiled. (B) Competition assay in which rNVLP were preincubated with the listed dilution amount of RE prior to incubation on PGM-coated plates. (C) rNVLP were preincubated with the listed amount of PGM prior to incubation on RE-coated plates. Error bars represent SD.

  • FIG. 4.
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    FIG. 4.

    Wells of a plate were coated with extracts from each of the different listed vegetables, and rNVLP binding was assayed by ELISA. Error bars represent SD.

  • FIG. 5.
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    FIG. 5.

    rNVLP binding to the surfaces of cilantro (A), iceberg lettuce (B), celery (C), and spinach (D) was assayed by confocal microscopy. Red, autofluorescence of vegetable; green, rNVLP.

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Binding of Virus-Like Particles of Norwalk Virus to Romaine Lettuce Veins
Kamal M. Gandhi, Robert E. Mandrell, Peng Tian
Applied and Environmental Microbiology Dec 2010, 76 (24) 7997-8003; DOI: 10.1128/AEM.01566-10

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Binding of Virus-Like Particles of Norwalk Virus to Romaine Lettuce Veins
Kamal M. Gandhi, Robert E. Mandrell, Peng Tian
Applied and Environmental Microbiology Dec 2010, 76 (24) 7997-8003; DOI: 10.1128/AEM.01566-10
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KEYWORDS

lettuce
Norwalk virus
Virus Attachment

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