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Methods

Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

Kilian Stoecker, Christiane Dorninger, Holger Daims, Michael Wagner
Kilian Stoecker
Department of Microbial Ecology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria
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Christiane Dorninger
Department of Microbial Ecology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria
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Holger Daims
Department of Microbial Ecology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria
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Michael Wagner
Department of Microbial Ecology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria
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  • For correspondence: wagner@microbial-ecology.net
DOI: 10.1128/AEM.02456-09
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Article Information

DOI 
https://doi.org/10.1128/AEM.02456-09
PubMed 
19966029

Published By 
American Society for Microbiology Journals
History 
  • Received October 9, 2009
  • Accepted November 25, 2009
  • Published online January 27, 2010.

Copyright & Usage 
Copyright © 2010 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (December 04, 2009).
  • You are viewing the most recent version of this article.

Author Information

  1. Kilian Stoecker,
  2. Christiane Dorninger,
  3. Holger Daims and
  4. Michael Wagner*
  1. Department of Microbial Ecology, University of Vienna, Althanstr. 14, 1090 Vienna, Austria
  • ↵* Corresponding author. Mailing address: University of Vienna, Department of Microbial Ecology, Althanstr. 14, 1090 Vienna, Austria. Phone: 43 1 4277 54390. Fax: 43 1 4277 54389. E-mail: wagner{at}microbial-ecology.net
  • † Supplemental material for this article may be found at http://aem.asm.org/ .

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Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility
Kilian Stoecker, Christiane Dorninger, Holger Daims, Michael Wagner
Applied and Environmental Microbiology Jan 2010, 76 (3) 922-926; DOI: 10.1128/AEM.02456-09

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Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility
Kilian Stoecker, Christiane Dorninger, Holger Daims, Michael Wagner
Applied and Environmental Microbiology Jan 2010, 76 (3) 922-926; DOI: 10.1128/AEM.02456-09
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KEYWORDS

Fluorescent Dyes
In Situ Hybridization, Fluorescence
Oligonucleotide Probes
RNA, Bacterial
RNA, Ribosomal

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