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Public Health Microbiology

Inactivation of Adenoviruses, Enteroviruses, and Murine Norovirus in Water by Free Chlorine and Monochloramine

Theresa L. Cromeans, Amy M. Kahler, Vincent R. Hill
Theresa L. Cromeans
1Atlanta Research and Education Foundation
2Centers for Disease Control and Prevention, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Division of Parasitic Diseases, Atlanta, Georgia 30341
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Amy M. Kahler
1Atlanta Research and Education Foundation
2Centers for Disease Control and Prevention, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Division of Parasitic Diseases, Atlanta, Georgia 30341
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Vincent R. Hill
1Atlanta Research and Education Foundation
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  • For correspondence: vhill@cdc.gov
DOI: 10.1128/AEM.01342-09
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  • FIG. 1.
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    FIG. 1.

    Free chlorine inactivation of CVB5 at pH 7 (▪) and pH 8 (□) and E1 at pH 7 (•) and pH 8 (○).

  • FIG. 2.
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    FIG. 2.

    Free chlorine inactivation of HAdV2 at pH 7 (▪) and pH 8 (□), CVB3 at pH 7 (•) and pH 8 (○), and E11 at pH 7 (▴) and pH 8 (▵).

  • FIG. 3.
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    FIG. 3.

    Monochloramine inactivation of HAdV40 at pH 7 (▪) and pH 8 (□), HAdV41 at pH 7 (•) and pH 8 (○), E1 at pH 7 (▴) and pH 8 (▵), and MNV at pH 7 (♦) and pH 8 (⋄).

  • FIG. 4.
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    FIG. 4.

    Monochloramine inactivation of CVB5 at pH 7 (▪) and pH 8 (□), CVB3 at pH 7 (•) and pH 8 (○), E11 at pH 7 (▴) and pH 8 (▵), and HAdV2 at pH 7 (♦) and pH 8 (⋄).

Tables

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  • TABLE 1.

    CT values for free chlorine inactivation of study viruses with 0.2 mg of free chlorine/liter at 5°C

    VirusCT value (mg·min/liter)
    2-log10 CT3-log10 CT4-log10 CT
    pH 7pH 8pH 7pH 8pH 7pH 8
    HAdV20.020.040.060.120.150.27
    HAdV40<0.02<0.02<0.02<0.02<0.04<0.04
    HAdV410.005<0.020.01<0.02NDc<0.03
    CVB30.970.651.41.12.91.7
    CVB53.64.75.57.67.4b10b
    E10.960.991.31.31.5a1.6
    E110.820.541.0a0.971.1a1.4a
    MNV<0.02<0.02<0.02<0.02<0.07<0.08
    • ↵ a CT value for this level of inactivation extrapolated using the EFH model.

    • ↵ b Only one replicate achieved the desired inactivation (the CT value for the second replicate was extrapolated).

    • ↵ c ND, no data (the CT value could not be extrapolated due to asymptotic inactivation curves).

  • TABLE 2.

    CT values for 2-, 3-, and 4-log10 inactivation of study viruses with 1 mg of monochloramine/liter at 5°C

    VirusCT value (mg·min/liter)
    2-log10 CT3-log10 CT4-log10 CT
    pH 7pH 8pH 7pH 8pH 7pH 8
    HAdV26009901,0001,6001,500b2,300
    HAdV4090360NDdNDNDND
    HAdV4158190190NDNDND
    CVB3270240390330500a420
    CVB55106707109009001,100
    E188151842a,cND
    E111,0008801,3001,2001,500a1,400b
    MNV26367078150170
    • ↵ a CT value for this level of inactivation extrapolated using the EFH model.

    • ↵ b Only one replicate achieved the desired inactivation (the CT value for the second replicate was extrapolated).

    • ↵ c Data from one replicate only.

    • ↵ d ND, no data. The CT value could not be extrapolated due to asymptotic inactivation curves.

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Inactivation of Adenoviruses, Enteroviruses, and Murine Norovirus in Water by Free Chlorine and Monochloramine
Theresa L. Cromeans, Amy M. Kahler, Vincent R. Hill
Applied and Environmental Microbiology Feb 2010, 76 (4) 1028-1033; DOI: 10.1128/AEM.01342-09

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Inactivation of Adenoviruses, Enteroviruses, and Murine Norovirus in Water by Free Chlorine and Monochloramine
Theresa L. Cromeans, Amy M. Kahler, Vincent R. Hill
Applied and Environmental Microbiology Feb 2010, 76 (4) 1028-1033; DOI: 10.1128/AEM.01342-09
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KEYWORDS

Chloramines
chlorine
enterovirus
Fresh Water
Norovirus
Water Purification

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